中国药房2018，Vol.29Issue(7)：930-932,3.DOI:10.6039/j.issn.1001-0408.2018.07.16
HPLC法测定绿芫菁中斑蝥素的含量
Content Determination of Cantharidin in Lytta caraganae by HPLC
摘要
Abstract
OBJECTIVE:To establish the method for content determination of cantharidin in Lytta caraganae,and to use the result as the extract screening evidence of L. caraganae source material. METHODS:Ultrasonic extraction method was used to extract cantharidin from L. caraganae using acetone as solvent. HPLC method was adopted to determine the content of cantharidin. The determination was performed on C18column with mobile phase consisted of methanol-water(23:77)at flow rate of 1.0 mL/min. The detection wavelength was set 230 nm,the column temperature was set at 35 ℃,and sample size was 10 μL. The content of cantharidin in L. caraganae was determined and compared with the results of content determination by the method stated in Chinese Pharmacopeia(using chloroform as extraction solvent). RESULTS:The liner range of cantharidin were 0.2-1.0 mg/mL (r=0.998 8)with average methodology recovery rates of 101.1%(RSD=1.7%,n=6). The average content of cantharidin in L. caraganae was 0.932%(n=3),while the content of cantharidin was 0.793%(n=3)determined by the method stated in Chinese Pharmacopeia. Both were higher than the requirement of Chinese Pharmacopeia that the content of cantharidin in scource material of cantharidin was higher than 0.35%. CONCLUSIONS:Established method is accurate and reliable for the content determination cantharidin in L. caraganae. The content of cantharidin is up to the standard of Chinese Pharmacopeia,and can be used as source material for exacting cantharidin.关键词
绿芫菁/斑蝥素/高效液相色谱法/丙酮提取/含量测定Key words
Lytta caraganae/Cantharidin/HPLC/Acetone extraction/Content determination分类
医药卫生引用本文复制引用
王腾蛟,赵成坚,谷颖乐,徐永莉,李力..HPLC法测定绿芫菁中斑蝥素的含量[J].中国药房,2018,29(7):930-932,3.基金项目
广西科学研究与技术开发计划项目(No.桂科能1598025-19) （No.桂科能1598025-19）
