中国药房2018，Vol.29Issue(7)：933-936,4.DOI:10.6039/j.issn.1001-0408.2018.07.17
气相色谱法同时测定夜寒苏药材挥发油中α-蒎烯、β-蒎烯及芳樟醇的含量
Simultaneous Determination of α-pinene,β-pinene and Linalool in Volatile Oil from Hedychium flavum by GC
摘要
Abstract
OBJECTIVE:To establish a method for simultaneous determination of α-pinene,β-pinene and linalool in volatile oil of Hedychium flavum. METHODS:The volatile oil was extracted from H. flavum according to steam distillation stated in Chinese Pharmacopoeia(2015 edition volume Ⅳ). GC method was adopted to determine the content. The determination was performed on HP-5 capillary chromatographic column(30 m×0.032 mm×0.25 μm)with hydrogen flame ion detector(FID),nitrogen gas as carrier gas,with injector temperature of 200 ℃,and detector temperature of 250 ℃,column flow rate of 0.8 mL/min, samples size of 1 μL and split ratio of 40:1 by programmed temperature. RESULTS:The linear range of α-pinene,β-pinene and linalool were 0.090 5-2.413 3 mg/mL(r=0.999 9),0.098 3-2.620 0 mg/mL(r=0.999 9),0.169 1-4.510 0 mg/mL(r=0.999 8), respectively. RSDs of precision,stability(12 h),reproducibility tests were no more than 2.0%(n=6). The average recoveries were 99.84%(RSD=0.49%,n=6),100.24%(RSD=1.38%,n=6),99.41%(RSD=1.67%,n=6),respectively.The contents of α-pinene,β-pinene and linalool ranged 0.214 4-1.325 0,0.766 2-3.172 1,0.357 4-1.518 7 mg/g in volatile oil from 23 batches of H. flavum produced in Guizhou province. CONCLUSIONS:The method established in this experiment is rapid,simple and accurate.It can be used for the content determination of α-pinene,β-pinene and linalool in volatile oil from H.flavum.关键词
夜寒苏/挥发油/α-蒎烯/β-蒎烯/芳樟醇/气相色谱法Key words
Hedychium flavum/Volatile oil/α-pinene/β-pinene/Linalool/GC分类
医药卫生引用本文复制引用
张彪,黄芳,周汉华,张浪,董明洪,于泽玥,张蜜..气相色谱法同时测定夜寒苏药材挥发油中α-蒎烯、β-蒎烯及芳樟醇的含量[J].中国药房,2018,29(7):933-936,4.基金项目
贵州省药监局基金(No.3040640150121) （No.3040640150121）
