中国药房2018，Vol.29Issue(7)：950-954,5.DOI:10.6039/j.issn.1001-0408.2018.07.21
龙眼叶不同极性部位对2型糖尿病小鼠的降血糖作用研究
Study on Hypoglycemic Effects of Different Polar Parts from the Leaves of Dimocarpus longan on Type 2 Diabetes Mellitus Mice
摘要
Abstract
OBJECTIVE:To optimize the hypoglycemic active parts from the leaves of Dimocarpus longan for type 2 diabetes mellitus mice,and to provide reference for material basis study of it. METHODS:Total extract was obtained from the leaves of D. longan with 95% ethanol percolation extraction. After confirming hypoglycemic effect of total extract,extract was suspended with water. Relevant extract was obtained by using petroleum ether,ethyl acetate and n-butanol in turn(respectively marked as ST,YT,ZT and WT). The model of type 2 diabetes mellitus mice was established by high glucose and high fat diet combined with STZ. Model mice were randomly divided into model group(distilled water),positive control group(metformin,0.1 g/kg), ST low-dose,medium-dose and high-dose groups(22.2,29.6,44 g/kg,calculated by crude drug,similarly hereinafter),YT low-dose,medium-dose and high-dose groups(10.7,16,32 g/kg),ZT low-dose,medium-dose and high-dose groups(10.5, 15.8,31.6 g/kg),WT low-dose,medium-dose and high-dose groups(18.5,24.6,37 g/kg),with 10 mice in each group. Other 10 normal mice were included in normal group(distilled water). Those groups were given relevant medicine intragastrically,once a day,for consecutive 28 d.The growth of mice and the change of body weight were observed and recorded. Fasting blood glucose(FBG)of mice was detected,and glucose tolerance test of mice was conducted. The contents of TC,TG,HDL-C and LDL-C in serum of mice were also determined. RESULTS:Compared with normal group,body weight and serum content of HDL-C in model group were decreased;FBG and the blood sugar after giving glucose for 0.5,1,2 h and area under glucose tolerance curve of mice were increased,and serum contents of TC,TG and LDL-C were alse increased,with statistical significance(P<0.01). compared with model group,body weight of mice in YT high-dose group after administration of 25 d was increased,FBG of mice in YT groups and ZT medium-dose,high-dose groups were decreased(P<0.05 or P<0.01);blood sugar of mice in YT medium-dose,high-dose groups and ZT high-dose group after giving glucose for 1 h as well as blood sugar of mice in YT high-dose group after giving glucose for 2 h were all decreased;area under glucose tolerance curve of mice and serum content of TC were decreased in YT groups and ZT high-dose group,while serum content of HDL-C was increased. Serum content of TG and LDL-C in YT high-dose group and ZT high-dose group as well as serum content of TG in YT medium-dose group were all decreased,serum content of HDL-C in ZT medium-dose group was increased,with statistical significance(P<0.05 or P<0.01). CONCLUSIONS:The ethyl acetate part and n-butanol part from the leaves of D. longan show good hypoglycemic effect on type 2 diabetes mellitus mice.关键词
龙眼叶/2型糖尿病/降血糖/不同极性部位/小鼠Key words
Leaves of Dimocarpus longan/Type 2 diabetes mellitus/Hypoglycemic/Different polar part/Mice分类
医药卫生引用本文复制引用
梁洁,金青青,黄团心,徐晖,麦嘉妮,信晨曦..龙眼叶不同极性部位对2型糖尿病小鼠的降血糖作用研究[J].中国药房,2018,29(7):950-954,5.基金项目
国家自然科学基金资助项目(No.81560691) （No.81560691）
广西中医药大学中药学博士点建设工程开放课题(No.201410-06) （No.201410-06）
2011协同创新中心(No.桂教科研〔2013〕20号) （No.桂教科研〔2013〕20号）
广西壮瑶药重点实验室(No.桂科基字〔2014〕32号) （No.桂科基字〔2014〕32号）
广西八桂学者中药创新理论与药效研究 （）
国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室 （壮）
广西高校中药提取纯化与质量分析重点实验室 （）
