中国医学前沿杂志（电子版）2018，Vol.10Issue(1)：32-36,5.DOI:10.12037/YXQY.2018.01-07
诱导C57BL/6小鼠骨髓单核细胞向破骨细胞分化的条件
The conditions of inducing C57BL/6 mouse bone marrow-derived macrophages differentiation into osteoclasts
摘要
Abstract
Objective To investigate the conditions inducing C57BL/6 mouse bone marrow-derived macrophages (BMMs) differentiation into osteoclasts, meanwhile increasing the efficiency of osteoclast induced from BMMs. Method Flow cytometer was used to detect the CD11b located in BMMs. The osteoclasts were induced form BMMs with macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor κB ligand (RANKL) for 4 days. And the osteoclasts were confirmed by tartrate-resistant acid phosphatase (TRAP) staining and phalloidin staining. The bone absorbent capacity of osteoclasts was measured by bone plate absorption test. Result The CD11b positive incidence detected by flow cytometer of BMMs was 98.4%. After 4 days inducing, BMMs turn into many osteoclasts by activating and fusing, which the TRAP staining was positive and phalloidin staining detect the fibrous actin ring. By means of microscope, a lot of anomalous bone resorption pits were found. Conclusion The osteoclasts that have powerful bone absorbent capacity are differentiated from BMMs with 4 days induction by RANKL.关键词
C57BL/6小鼠骨髓单核细胞/破骨细胞/巨噬细胞集落刺激因子/核因子κB受体活化因子配体Key words
C57BL/6 mouse bone marrow-derived macrophages/Osteoclast/Macrophage colony-stimulating factor/Receptor activator of nuclear factor κB ligand引用本文复制引用
曾立,耿欢,刘水涛,姜川,邢更彦..诱导C57BL/6小鼠骨髓单核细胞向破骨细胞分化的条件[J].中国医学前沿杂志（电子版）,2018,10(1):32-36,5.基金项目
国家自然科学基金(31172169) （31172169）
