中国中医药信息杂志2018，Vol.25Issue(4)：28-34,7.DOI:10.3969/j.issn.1005-5304.2018.04.007
不同中医证型原发性失眠患者肠道菌群差异研究
Study on Difference of Intestinal Flora in Patients with Primary Insomnia with Different TCM Syndromes
摘要
Abstract
Objective To explore structural differences of intestinal flora in primary insomnia patients with different TCM syndromes through the high-throughput 16S rDNA sequencing analysis. Methods Totally 65 patients with primary insomnia were divided into 22 patients with syndrome of liver depression transforming into fire, 17 patients with deficiency of both heart and spleen syndrome, 26 patients with syndrome of hyperactivity of fire due to yin deficiency, with 47 cases of healthy people as the control group. The fecal flora structure of the subjects was analyzed by high-throughput 16S rDNA sequencing. QIIME software and R language stats package were used to analyze the diversity of flora. Results Totally 1226 different operational taxonomic units (OUTs) were obtained, and there were 180 significant differences among the 4 groups (P<0.05), indicating that the samples were rich in microbial colonies. The mapped reads in group of liver depression transforming into fire and hyperactivity of fire due to yin deficiency were more than the group of deficiency of both heart and spleen and the control group (P<0.05). Unweighted UniFrac analysis showed that the difference among groups was remarkably greater than the difference within group, and the grouping was statistically significant (R=0.103, P=0.002). It suggested that the diversity of intestinal flora was highly correlated with different TCM syndromes of insomnia. There were a total of 57 genera found significant differences among the different groups at the genus level (P<0.05), and 115 species at all species level. The dominant flora of the control group were prevotella, megamonas, clostridium Ⅺ (clostridium ⅩⅧ), weissella, and alloprevotella; The dominant flora of liver depression transforming into fire syndrome were phascolarctobacterium, flavonifractor, eggerthella, and bilophila; The dominant flora of deficiency of both heart and spleen syndrome were sphingomonas and methylobacterium; The dominant flora in hyperactivity of fire due to yin deficiency syndrome group were bacteroides, parabacteroides, parasutterella, butyricimonas, odoribacter. Conclusion The patients with primary insomnia have abundant intestinal flora diversity and diverse flora structure, which may affect the occurrence, development and outcome of different TCM syndromes.关键词
原发性失眠/中医证候/16S rDNA测序/肠道菌群Key words
primary insomnia/TCM syndromes/16S rDNA sequencing/intestinal flora分类
医药卫生引用本文复制引用
骆杰伟,吴永希,黄昉萌,陈炜玮,朱耀彬,魏世超,郑星宇,杨笑..不同中医证型原发性失眠患者肠道菌群差异研究[J].中国中医药信息杂志,2018,25(4):28-34,7.基金项目
福建省医药卫生科技创新项目(2015-CX-4) （2015-CX-4）
福建省卫生系统中青年骨干人才培养项目资助计划(2015-ZQN-ZD-7) （2015-ZQN-ZD-7）
福建省卫生计生青年科研课题(2016-1-84) （2016-1-84）
