中国中医药信息杂志2018，Vol.25Issue(4)：66-70,5.DOI:10.3969/j.issn.1005-5304.2018.04.014
一测多评法测定黄蛭内异胶囊中5种蒽醌类成分含量
Content Determination of 5 Anthraquinones in Huangzhi Neiyi Capsules by QAMS
摘要
Abstract
Objective To determine the contents of 5 anthraquinones (aloe-emodin, phein, emodin, chrysophanol, physcion)in Huangzhi Neiyi Capsule through a quantitative analysis of multi-component by single-marker(QAMS), To determine the application value of QAMS by comparing with external standard method (ESM). Methods HPLC was performed at 30 ℃ with Waters Symmetry C18 column (4.6 mm × 250 mm, 5 μm), methanol-0.4% phosphoric acid (85:15) as mobile phase at the wavelength 440 nm, and flow rate of 1.000 mL/min. The relative correction factors of other anthraquinones were calculated respectively by emodin as the reference. Results The values calculated by QAMS were compared with the values measured by ESM, and no significant difference was showed (RSD<5%). Conclusion For the availability and reliability, QAMS can be applied for quality evaluation of Huangzhi Neiyi Capsules.关键词
一测多评法/黄蛭内异胶囊/蒽醌类/含量测定Key words
quantitative analysis of multi-component by single-marker/Huangzhi Neiyi Capsules/anthraquinones/content determination分类
医药卫生引用本文复制引用
徐冲,沈洁,夏敏,杨敏,冷静..一测多评法测定黄蛭内异胶囊中5种蒽醌类成分含量[J].中国中医药信息杂志,2018,25(4):66-70,5.基金项目
重庆市社会事业与民生保障科技创新专项重点项目(cstc2016shms-zd10001) （cstc2016shms-zd10001）
重庆市中医院院内培育课题(2016-1-10) （2016-1-10）
