中华肝脏外科手术学电子杂志2018，Vol.7Issue(2)：147-151,5.DOI:10.3877/cma.j.issn.2095-3232.2018.02.015
端粒酶调控因子PinX1抑制肝癌细胞增殖与侵袭
Telomerase regulation factor PinX1 inhibits proliferation and invasion of hepatoma cells
摘要
Abstract
Objective To explore the impact of telomerase regulation factor PinX1 to the proliferation and invasion ability of hepatoma cells. Methods Hepatoma cells PinX1-7721 (experimental group) with stable expression of PinX1 as well as control cell VECTOR-7721 (control group) were constructed. The expression of PinX1 mRNA was detected by RT-PCR. The proliferation ability and clonality of hepatoma cells were detected by CCK-8 method and plate clonality assay, and the invasion ability of hepatoma cells by Transwell assay. Comparison of the experiment data was conducted by t test. Results Expression level of PinX1 mRNA in experiment group was (13.9±2.0)×10-3, which was significantly higher than (1.1±0.2)×10-3in control group (t=10.98, P<0.05). A450of the cells on 1-7 d in experiment group was respectively 0.260±0.004, 0.340±0.008, 0.450±0.040, 0.500±0.020, 0.730±0.030, 1.350±0.040 and 1.640±0.050, which were significantly lower than 0.280±0.009, 0.410±0.007, 0.680±0.044, 0.730±0.029, 0.850±0.070, 1.700±0.020 and 2.080±0.280 in control group (t=-5.82, -12.99, -6.36, -5.96, -28.42,-18.98, -5.08; P<0.05). The plate clonality assay results showed that the clone formation quantity of cells in experiment group was 143±32, which was significantly lower than 305±25 in control group (t=-6.91, P<0.05).Transwell assay results showed that the quantity of trans-membrane cell in experiment group was 230±16, which was significantly lower than 650±30 in control group (t=-21.40, P<0.05). Conclusion PinX1 could inhibit the proliferation and invasion ability of hepatoma cells.关键词
癌,肝细胞/端粒酶/PinX1/hTERTKey words
Carcinoma,hepatocellular/Telomerase/PinX1/hTERT引用本文复制引用
李瑞曦,姚志成,熊志勇,周伯宣,徐见亮,胡昆鹏,范伟明,梁豪,邓美海..端粒酶调控因子PinX1抑制肝癌细胞增殖与侵袭[J].中华肝脏外科手术学电子杂志,2018,7(2):147-151,5.基金项目
广东省自然科学基金(2016A030313848,S2013010016015) （2016A030313848,S2013010016015）
广州市科技计划项目(2013J4100061) （2013J4100061）
