作物学报2018,Vol.44Issue(4):473-482,中插1-中插5,15.DOI:10.3724/SP.J.1006.2018.00473
中国小麦品种抗赤霉病基因Fhb1的鉴定与溯源
Characterization of Fusarium Head Blight Resistance Gene Fhb1 and Its Puta-tive Ancestor in Chinese Wheat Germplasm
摘要
Abstract
Enhancing resistance to Fusarium head blight (FHB)has become one of important breeding objectives in the major wheat-growing regions in China.A prominent locus Fhb1 conferring stable FHB resistance with the largest effect is the major source of resistance in wheat breeding.Understanding the distribution and putative donor of Fhb1 in Chinese wheat cultivars will facilitate the application of this gene and thus benefit FHB resistance breeding in China.Haplotype analysis of PFT(pore-forming toxin-like),HC(HCBT-like defense response protein)and His(histidine-rich calcium-binding protein)genes in the Fhb1 region of 229 wheat cultivars and advanced lines indicated that PFT-I/His-I was a resistant haplotype. Both pedigree and marker (or se-quence)information revealed that Fhb1 in Chinese wheat cultivars was mainly derived from Sumai 3 and Ningmai 9,in which Ningmai 9 was the major donor. The Fhb1 diagnostic markers PFT-CAPS and His-InDel developed in this study can be used effectively in marker-assisted selection for improving FHB resistance.关键词
小麦/赤霉病/Fhb1/诊断性标记/分子标记辅助选择Key words
bread wheat/Fusarium head blight/Fhb1/diagnostic marker/marker-assisted selection引用本文复制引用
朱展望,徐登安,程顺和,高春保,夏先春,郝元峰,何中虎..中国小麦品种抗赤霉病基因Fhb1的鉴定与溯源[J].作物学报,2018,44(4):473-482,中插1-中插5,15.基金项目
本研究由国家重点研发计划项目(2016YFD0101802,2016YFE0108600),国家自然科学基金项目(31301306)和湖北省技术创新专项(2016AHB022)资助.This study was supported by the National Key Research and Development Program of China(2016YFD0101802,2016YFE0108600),Na-tional Natural Science Foundation of China(31301306),and the Technology Innovation Program of Hubei Province(2016AHB022). (2016YFD0101802,2016YFE0108600)