作物学报2018，Vol.44Issue(4)：483-492,10.DOI:10.3724/SP.J.1006.2018.00483
BnA7HSP70分子伴侣结合蛋白超表达能够提高甘蓝型油菜耐旱性
Enhanced Accumulation of BnA7HSP70 Molecular Chaperone Binding Protein Improves Tolerance to Drought Stress in Transgenic Brassica napus
摘要
Abstract
The molecular chaperone binding protein gene participates in the constitutive function of plant growth and protects plant cells against stresses.In this study,we found that BnA7HSP70 overexpressed transgenic lines did not wilt and showed only a small decrease in water potential. However, the wild type lines showed a large decrease in leaf water potential. The transgenic plants had higher relative water content, better osmotic adjustment and less lipid membrane peroxidation. In addition, the leaves from the elevated levels of BnA7HSP70 in transgenic lines conferred tolerance to the glycosylation inhibitor tunicamycin during germination.BnA7HSP70 overexpression-mediated attenuation of stress-induced cell death was confirmed by the decreased per-centage of dead cell and the reduced induction of the senescence-associated marker gene BnCNX1. These phenotypes were accompanied by a delay in the induction of the cell death marker genes BnNRP,which are involved in transducing a cell death signal generated by ER stress and osmotic stress through the NRP (N-rich protein)-mediated signaling pathway. Enhanced expres-sion of BnA7HSP70 delayed unfold protein response and NRP pathway mediated chlorosis and the appearance of senes-cence-associated markers BnLSC222 and BnLSC54 in Brassica napus.These results suggest that overexpression of BnA7HSP70 in Brassica napus alleviate ER stress and osmotic stress-integrating cell death response confronted with water stress.关键词
BnA7HSP70/耐旱性/甘蓝型油菜/叶片衰老/抗氧化酶Key words
BnA7HSP70/drought tolerance/Brassica napus/leaf senescence/antioxidant enzymes引用本文复制引用
万丽丽,王转茸,辛强,董发明,洪登峰,杨光圣..BnA7HSP70分子伴侣结合蛋白超表达能够提高甘蓝型油菜耐旱性[J].作物学报,2018,44(4):483-492,10.基金项目
本研究由湖北省武汉市农业科学院特种经济作物种质资源创新与产业开发技术研究项目(CX201710),国家自然科学基金青年科学基金项目(31401413)和湖北省重大技术创新项目(2016ABA084)资助.This study was supported by Germplasm Innovation Foundation of Wuhan Academy of Agricultural Sciences(CX201710),the National Natural Science Foundation of China(31401413),and the High Technology Innovation Foundation of Hubei Province(2016ABA084). （CX201710）
