作物学报2018，Vol.44Issue(4)：493-504,12.DOI:10.3724/SP.J.1006.2018.00493
玉米光合突变体hcf136(high chlorophyll fluorescence 136)的转录组分析
Transcriptome Analysis on a Maize Photosynthetic Mutant hcf136 (high chlo-rophyll fluorescence 136)
摘要
Abstract
Maize (Zea mays) is a typical C4plant, which photosynthetic activities are partitioned by the cooperating of Kranz anatomy—mesophyll(M)cell and bundle sheath(BS)cell.The maize high chlorophyll fluorescence 136(hcf136)mutant is an ideal material for C4photosynthesis study, due to it lacks PSII activity without normal thylakoid grana in M chloroplasts. However, the development of BS chloroplasts is not affected. In this study, we utilized RNA-Seq technology to monitor the transcriptome changes in different leaf sections between wild type and hcf136 mutant under different light intensities.The results indicated that the impairment of PSII is not caused by the transcription changes of PSII related genes. We also noticed that the starch biosynthe-sis was blocked in the mutant; and the sugar degradation, sugar transport and copper ion transport were triggered. In addition, the expression level of transcription factors was changed distinctly. These results provide valuable information for understanding a comprehensive function of HCF136 gene in future.关键词
玉米/hcf136突变体/RNA-Seq/C4光合Key words
Zea mays/high chlorophyll fluorescence 136 mutant/RNA-Seq/C4photosynthesis引用本文复制引用
吴庆飞,秦磊,董雷,丁泽红,李平华,杜柏娟..玉米光合突变体hcf136(high chlorophyll fluorescence 136)的转录组分析[J].作物学报,2018,44(4):493-504,12.基金项目
本研究由国家自然科学基金项目(31271393)资助.This study was supported by the National Natural Science Foundation of China(31271393). （31271393）
