作物学报2018，Vol.44Issue(4)：505-511,7.DOI:10.3724/SP.J.1006.2018.00505
利用Fhb1基因功能标记选择提高黄淮冬麦区小麦品种对赤霉病的抗性
Improvement of Resistance of Wheat Cultivars to Fusarium Head Blight in the Yellow–Huai Rivers Valley Winter Wheat Zone with Functional Marker Selec-tion of Fhb1 Gene
摘要
Abstract
Fusarium head blight(FHB)becomes a major disease in the wheat production of the Yellow–Huai Rivers Valley Winter Wheat Zone of China.Improving FHB resistance is one of the major breeding targets in this region.The donors of Fhb1 gene,including Ningmai 9, Shengxuan 6, Jianyang 798, Jianyang 84, Sumai 3, and Ningmai 13, were moderately or highly resistant to FHB. Six backcrossing populations were developed by crossing these donors with a dwarfing and male-sterile wheat near isogenic line Zhoumai 16, and backcrossing with Zhoumai 16. The progenies were subjected to phenotypic evaluation of FHB by both the floret-inoculation method and natural infection in a field disease nursery.Fhb1 was detected by a functional Kompetitive Allele Specific PCR(KASP)marker.The backcrossing progenies with Fhb1 gene were moderately susceptible to FHB.Compared with the backcrossing progenies without Fhb1 gene,mean number of diseased spikelets and disease index reduced by 4.2(P<0.01) and 4.0 in the progenies with Fhb1 gene, respectively. The mean number of diseased spikelets and disease index for the back-crossing progenies with Fhb1 gene were 8.1(P<0.01)and 28.4(P<0.01)lower than those of the recurrent parent Zhoumai 16. There were significant differences in resistance among the backcrossing progenies from different donors. The progenies from Shengxuan 6 had better performance in number of diseased spikelets and disease index compared with those from any other do-nors.The results from this study indicate that Fhb1 gene can efficiently improve the FHB resistance of wheat cultivars grown in the Yellow–Huai Rivers Valley Winter Wheat Zone.关键词
小麦/赤霉病抗性/Fhb1基因/分子标记辅助选择Key words
Triticum aestivum L./Fusarium head blight resistance/Fhb1 gene/molecular marker-assisted selection引用本文复制引用
张宏军,刘宏伟,杨丽,李洪杰,周阳,宿振起,柏贵华,张旭,马鸿翔,李腾,邓云,买春艳,于立强..利用Fhb1基因功能标记选择提高黄淮冬麦区小麦品种对赤霉病的抗性[J].作物学报,2018,44(4):505-511,7.基金项目
本研究由国家重点研发计划项目(2016YFD0101802,2017YFD0100600)和作物分子育种国家工程实验室项目资助.This study was supported by the National Key Research and Development Program of China(2016YFD0101802,2017YFD0100600)and the National Engineering Laboratory of Crop Molecular Breeding. （2016YFD0101802,2017YFD0100600）
