作物学报2018，Vol.44Issue(4)：542-553,12.DOI:10.3724/SP.J.1006.2018.00542
草铵膦胁迫下油菜苗期叶片药害相关性状的全基因组关联分析
Genome-wide Association Analysis of Some Phytotoxicity Related Traits at Seedling Stage in Rapeseed under Glufosinate Stress
摘要
Abstract
With planting rape in large area and weeds growing seriously in the field, glufosinate has been used in controlling field weeds tentatively. However glufosinate also produced phytotoxicity to the main cultivated rapeseed more or less, affecting the normal physiological and morphological activities, even yield. In this study, glufosinate of 200 mg L–1was used at stress concen-tration 506 representative Brassica napus varieties(lines)were genotyped using the Brassica 60k Illumina Infinium SNP array by genome-wide association studies of herbicide resistance coefficient of dry weight per unit leaf area (CLW), herbicide resistance coefficient of chlorophyll content (CCC) and comprehensive phytotoxicity coefficient (CPC). A total of 16 SNP sites related to the killing mechanism of glufosinate were detected. Six SNP sites associated with CLW were detected, with phenotypic contribution rate ranging from 6.53% to 10.04%, and 22 SNP sites associated with CCC, with the phenotypic from 4.97% to 6.20%, 98 SNP sites associated with CPC over A subgenome and C subgenome, with the rate range from 3.25% to 18.66%. After analysising the genes in the linkage disequilibrium (LD) interval of significant SNP, we found 18 glufosinate tolerance related candidate genes. Eleven candidate genes were found in the LD interval of SNPs related to CCC, among them nine were involved in acyltransferase activity, two were involved in acetyl-CoA transferase activity, seven candidate genes were found in the LD interval of SNPs re-lated to CPC, one of them was associated with glutamine transferase activity, participating in glutamine metabolism, and other six genes were involved in acetyl CoA transferase activity. The physiological and biochemical process regulated by these genes re-lated to the changes of leaf dry weight, chlorophyll content and the killing mechanism of glufosinate. Mining association sites and candidate genes provides a reference for the research of gene regulation mechanism in the physiological and morphological proc-ess under glufosinate stress.关键词
油菜/苗期/草铵膦/全基因组关联分析Key words
Brassica napus L./seedling stage/glufosinate/genome-wide association studies引用本文复制引用
陈东亮,崔翠,任义英,王倩,李加纳,唐章林,周清元..草铵膦胁迫下油菜苗期叶片药害相关性状的全基因组关联分析[J].作物学报,2018,44(4):542-553,12.基金项目
本研究由国家科技支撑计划项目(2013BAD01B03-12),国家现代农业产业技术体系建设专项(CARS-12),重庆市社会事业与民生保障科技创新(cstc2016shmszx0756)和西南大学博士启动基金(swu113064)资助.This study was supported by the National Key Technology Support Program of China(2013BAD01B03-12),the China Agriculture Research System(CARS-12),the Science and Technology Committee of Chongqing(cstc2016shmszx0756),and the Doctoral Start-up Fund of South-western University(swu113064). （2013BAD01B03-12）
