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白桦BpBEE1基因启动子的表达特性分析

许思佳 颜斌 董京祥 武丹阳 李慧玉

植物研究2018,Vol.38Issue(2):247-253,7.
植物研究2018,Vol.38Issue(2):247-253,7.DOI:10.7525/j.issn.1673-5102.2018.02.012

白桦BpBEE1基因启动子的表达特性分析

Analysis of BpBEE1 Gene Promoter in Betula platyphylla

许思佳 1颜斌 1董京祥 2武丹阳 1李慧玉1

作者信息

  • 1. 东北林业大学林木遗产育种国家重点实验室,哈尔滨150040
  • 2. 东北林业大学,哈尔滨150040
  • 折叠

摘要

Abstract

Brassinolide Enhanced Expression (BEE) genes belong to bHLH transcription factors family,and were involved in Brassinolide signal pathway.In this study,the promoter of BpBEE1 gene was cloned,and regulatory elements in the promoter regions were analyzed using the online program PLACE.The results showed that the BEE1 promoter sequence contained various hormone responses elements.The results showed that the GUS activity was increased in the veins and roots,and the activity of promoter was enhanced after MeJA,SA,BL and ABA treatment,especially MeJA,SA and BL treatment.We propose that BpBEE1 is involved in hormone response,and play a role in the process of plant growth and development.

关键词

白桦/BpBEE启动子/表达特性/激素

Key words

Betula platyphylla/BpBEE promoter/Genetic transformation/honmones

分类

农业科技

引用本文复制引用

许思佳,颜斌,董京祥,武丹阳,李慧玉..白桦BpBEE1基因启动子的表达特性分析[J].植物研究,2018,38(2):247-253,7.

基金项目

国家重点研发计划(2017YFD0600603)National Key R&D Plan(2017YFD0600603) (2017YFD0600603)

植物研究

OA北大核心CSCDCSTPCD

1673-5102

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