植物研究2018，Vol.38Issue(2)：254-259,6.DOI:10.7525/j.issn.1673-5102.2018.02.013
拟南芥转录共激活子ANGUSTIFOLIA3(AN3)调控花的雄蕊的形成
Arabidopsis thaliana Transcription Coactivator ANGUSTIFOLIA3 (AN3) in Regulating the Stamen Formation
摘要
Abstract
Stamens are important reproductive organs which produce pollen in seed plants,and their normal development is related to the reproductive status of plants and affects the yield of crops.Two knockout mutants an3-1 and an3-4 of Arabidopsis thaliana transcription coactivator ANGUSTIFOLIA3 (AN3) were identified by RT-PCR.By morphological examination,the stamens of the an3-1 and an3-4 mutants were shorter than those of wild-type,whereas,the pistils were not changed significantly.By constructing AN3 promoter with GUS expression vector,the flower tissues of obtained Pro-AN3-GUS plants were stained and observed.The AN3 promoter in Arabidopsis seed embryos,mature pollen,stigma,petals was expressed.AN3 could be expressed in important tissues such as floral organs during Arabidopsis reproductive growth.These above results are consistent with the short stamens of an3-1 and an3-4 mutants.Thus,AN3 positively regulates the stamen development of A.thaliana.关键词
ANGUSTIFOLIA3(AN3)/突变体/雄蕊/调控Key words
ANGUSTIFOLIA3 (AN3)/mutant/stamens/regulation分类
农业科技引用本文复制引用
李丹,徐梦珂,蒋继宏,孟来生..拟南芥转录共激活子ANGUSTIFOLIA3(AN3)调控花的雄蕊的形成[J].植物研究,2018,38(2):254-259,6.基金项目
徐州市农业科技项目(农业高技术攻关项目KC16NG063)Agricultural science and technology(agricultural high-tech research project)(KC16NG063) （农业高技术攻关项目KC16NG063）
