植物研究2018，Vol.38Issue(2)：268-277,10.DOI:10.7525/j.issn.1673-5102.2018.02.015
2个慈竹bZIP基因的克隆、生物信息学分析及其诱导表达
Cloning and Bioinformatics Analysis of Two bZIP Genes of Bambusa emeiensis and Their Induced Expression under Abiotic Stresses
摘要
Abstract
According to the transcriptome data of Bambusa emeiensis shoot,two bZIP genes designated as BebZIP2 and BebZIP6 were cloned and their bioinformatics were analyzed.The bioinformatics analysis results showed that the full-length cDNA sequence of BebZIP2 and BebZIP6 were 504 and 720 bp,and encoded 167 and 239 amino acids,respectively.BebZIPs and rice OsbZIP52/RISB5 proteins were clustered into the same branch,which were bZIP-related proteins.The tissue expression patterns of two BebZIP genes were analyzed by real-time PCR.Two BebZIP gene sequences were expressed in all shoots,stalks,unfolding leaves and rolled leaves of B.emeiensis.There were the expression differences of the same gene in the different tissues and expression quantity in the descending order was unfolding leaves,rolled leaves,stalks and shoots.The seedlings of B.emeiensis were treated with ABA,NaCl and PEG 6000 for abiotic stress analysis.The expression level of BebZIP2 and BebZIP6 genes treated by salt,drought and ABA stresses have different degree of sensitivity.关键词
慈竹/bZIP转录因子/生物信息学分析/组织表达分析/非生物胁迫Key words
Bambusa emeiensis/bZIP transcription factors/bioinformatics analysis/tissue expression analysis/abiotic stresses分类
农业科技引用本文复制引用
龚道勇,胡尚连,曹颖,卢学琴,张庆波..2个慈竹bZIP基因的克隆、生物信息学分析及其诱导表达[J].植物研究,2018,38(2):268-277,10.基金项目
国家自然科学基金(31400333,31400257) （31400333,31400257）
四川省“十三五”重点攻关项目(2016NYZ0038) （2016NYZ0038）
四川省重点研发项目(2017NZ0008) （2017NZ0008）
西南科技大学研究生创新基金项目(17ycx086)National Natural Science Foundation of China (31400333,31400257) （17ycx086）
Sichuan Province's 13th Five Key Breeding Project (2016NYZ0038) （2016NYZ0038）
Key Research and Development Project of Sichuan Province,China(2017NZ0008) （2017NZ0008）
Graduate Innovation Project of Southwest University of Science and Technology(17ycx086) （17ycx086）
