植物研究2018，Vol.38Issue(2)：306-315,10.DOI:10.7525/j.issn.1673-5102.2018.02.020
利用荟萃分析挖掘拟南芥低温胁迫响应基因
Meta-analysis Identify Candidate Genes for Cold Stress Response in Arabidopsis
摘要
Abstract
Cold stress is one of the major environmental limitations to agricultural productivity throughout the world.Overcoming the defects of microarray sequencing analyses caused by heterogeneity in individual studies and prediction of cold stress response candidate genes and pathways in Arabidopsis thaliana are requisite for cold tolerant crop-plant design and mining potential salt responses mechanisms.A literature searching strategy was used to identify salt stress response expression profiling studies in Arabidopsis from the PubMed database,the Gene Expression Omnibus (GEO) database,and the ArrayExpress database.Eight studies were filtered from the 1 166,298 and 695 identified studies.The batch effect correction and the effect size analysis based on the NetworkAnalyst web tool were combined to integrate 8 microarray datasets,and 1 553 up-regulated and 4 327 down-regulated were identified with in low temperature treated samples.From the functional enrichment analyses results and the differently spatio-temporal expression patterns of transcriptional factors (TFs),protein kinases (PKs) and several plant hormone-related genes,plant hormone related stress signal construction pathways,ion and protein kinases related signaling pathways and transcriptional regulation were predicted to participate in regulating cold stress response in Arabidopsis.关键词
冷胁迫/拟南芥/荟萃分析/微阵列芯片/NetworkAnalystKey words
cold stress/Arabidopsis thanliana/meta-analysis/microarray/NetworkAnalyst分类
生物科学引用本文复制引用
段本耀,张学杰,樊守金,张洛艳..利用荟萃分析挖掘拟南芥低温胁迫响应基因[J].植物研究,2018,38(2):306-315,10.基金项目
国家自然科学基金(31470298和31170173) （31470298和31170173）
山东省科技发展专项(2010GSF10205)Natural Science Foundation of China (31470298 and 31170173) （2010GSF10205）
Science and Technology Development Foundation of Shandong Province (2010GSF10205) （2010GSF10205）
