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越橘茎尖离体培养条件的研究

李艳霞 邓继峰 李桂君

植物研究2018,Vol.38Issue(2):316-320,5.
植物研究2018,Vol.38Issue(2):316-320,5.DOI:10.7525/j.issn.1673-5102.2018.02.021

越橘茎尖离体培养条件的研究

The in vitro Culture for Vaccinium vitis-idaea Stem Apex

李艳霞 1邓继峰 2李桂君1

作者信息

  • 1. 黑龙江省林业科学研究所,哈尔滨150081
  • 2. 沈阳农业大学林学院,沈阳110866
  • 折叠

摘要

Abstract

In order to improve the breeding rate and qualities of Vaccinium vitis-idaea seedlings,in our study,V.vitis-idaea stem apexes were used as research materials,then rapid propagation systems of plants induced bystem apex in vitro culture was established.The results indicated that the optimal induced stem apex in vitro culture is WPM (improved) + ZT 0.05 mg · L-1 + IBA 0.5 mg · L-1 + GA30.1 mg · L-1.The success rate of plants induced by 0.1-0.3 mm long stem apex was only 28%,while such a rate of plants induced by 0.3-0.5 mm long stem apex was up to 68%;then stem apex reached to 0.5-0.7 mm,the success rate was 79%,and the success rate of plants induced by 0.7-1.0 mm long stem apex reached up to 94%.

关键词

越橘/茎尖培养/激素/培养基

Key words

Vaccinium vitis-idaea L./stem apex culture/hormone/culture medium

分类

农业科技

引用本文复制引用

李艳霞,邓继峰,李桂君..越橘茎尖离体培养条件的研究[J].植物研究,2018,38(2):316-320,5.

基金项目

黑龙江省森工总局项目(sgzjY2014008) (sgzjY2014008)

中国—白俄罗斯政府间科技合作项目(CB11-15)Heilongjiang provincial forestry bureau program (sgzjY2014008) (CB11-15)

China-Belarus-science and technology cooperation project (CB11-15) (CB11-15)

植物研究

OA北大核心CSCDCSTPCD

1673-5102

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