植物研究2018,Vol.38Issue(2):316-320,5.DOI:10.7525/j.issn.1673-5102.2018.02.021
越橘茎尖离体培养条件的研究
The in vitro Culture for Vaccinium vitis-idaea Stem Apex
摘要
Abstract
In order to improve the breeding rate and qualities of Vaccinium vitis-idaea seedlings,in our study,V.vitis-idaea stem apexes were used as research materials,then rapid propagation systems of plants induced bystem apex in vitro culture was established.The results indicated that the optimal induced stem apex in vitro culture is WPM (improved) + ZT 0.05 mg · L-1 + IBA 0.5 mg · L-1 + GA30.1 mg · L-1.The success rate of plants induced by 0.1-0.3 mm long stem apex was only 28%,while such a rate of plants induced by 0.3-0.5 mm long stem apex was up to 68%;then stem apex reached to 0.5-0.7 mm,the success rate was 79%,and the success rate of plants induced by 0.7-1.0 mm long stem apex reached up to 94%.关键词
越橘/茎尖培养/激素/培养基Key words
Vaccinium vitis-idaea L./stem apex culture/hormone/culture medium分类
农业科技引用本文复制引用
李艳霞,邓继峰,李桂君..越橘茎尖离体培养条件的研究[J].植物研究,2018,38(2):316-320,5.基金项目
黑龙江省森工总局项目(sgzjY2014008) (sgzjY2014008)
中国—白俄罗斯政府间科技合作项目(CB11-15)Heilongjiang provincial forestry bureau program (sgzjY2014008) (CB11-15)
China-Belarus-science and technology cooperation project (CB11-15) (CB11-15)