安徽农业科学2018，Vol.46Issue(5)：7-9,24,4.
响应面法优化青蛤降血压肽酶解制备工艺
Optimization of Enzymatic Hydrolysis Process for Preparation of Antihypertensive Peptides from Cyclina sinensis by Response Surface Methodology
摘要
Abstract
[Objective] Enzymatic hydrolysis process for preparing antihypertensive peptides from Cyclina sinensis was optimized by using response surface methodology.[Method] Taking angiotensin-converting enzyme (ACE) inhibition rate as index,a 3-factor and 3-level Box-Behnken design coupled with response surface analysis was carried out to investigate the effects of pH,hydrolysis time and solid-liquid ratio on ACE inhibition rate.[Result] The optimal conditions for preparing antihypertensive peptides from Cyclina sinensis by using papain were as followed:pH 5.8,hydrolysis time 8.4 h and solid-liquid ratio1:3.1,and the ACE inhibition rate of enzymatic hydrolysates were up to 93.58％ under these conditions.[Conclusion] This study provides novel ideas for the high-value use of Cyclina sinensis and preparing antihypertensive peptides.关键词
青蛤/木瓜蛋白酶/降血压肽/响应面法Key words
Cyclina sinensis/Papain/Antihypertensive peptides/Response surface methodology分类
农业科技引用本文复制引用
詹秋桂,李杰煌,施鹏飞,雷鸣,罗李王,余方苗..响应面法优化青蛤降血压肽酶解制备工艺[J].安徽农业科学,2018,46(5):7-9,24,4.基金项目
浙江省大学生科技创新项目(2015R411046) （2015R411046）
浙江省教育厅项目(Y201534400). （Y201534400）
