安徽农业科学2018，Vol.46Issue(5)：117-119,3.
西藏圆齿红景天茎内曲霉属真菌系统发育分析
Phylogenetic Analysis of Aspergillus Fungus in the Stem of Rhodiola crenulata of Tibet
向明学 1金鹏杰 2段双全 1许鹏辉 1拉多1
作者信息
- 1. 西藏大学,西藏拉萨850000
- 2. 拉萨北京实验中学,西藏拉萨850000
- 折叠
摘要
Abstract
[Objective] The morphological observations of Aspergillus strains isolated from the stem of the healthy fresh Tibet crassulaceae plants were studied and the separation and identification were conducted from the molecular biological level.[Method] The determination of the strains and the biological characteristics of the strain were carried out with the method of carrying plate culture,and the evolution tree analysis was established using the rRNA gene ITS interval region,and the system development of the fungus was elucidated.[Result] Colony in modified form KB medium and form was consistent in PDA medium,at first colony for white villous,edge column flake,dense and opaque,mycelium thick at base fiat provides quality,grooving villous strains for asexual.The isolated strain of the evolutionary tree were identified as the yellow-green group fungus of Aspergillus by the molecular biology of the rRNA gene ITS interval regional system development.[Conclusion] Through the observation and analysis of the external morphology of the colony and the phylogenetic analysis of the ITS spacer region of the asexual rRNA gene from the point of view of molecular biology,it is known that the isolated strain belongs to the fungus of the yellow-green group of Aspergillus.关键词
圆齿红景天/曲霉属黄绿组/系统发育/ITS间隔分析/西藏Key words
Rhodiola crenulata/Yellow and green groups of Aspergillus/Phylogeny/ITS interval analysis/Tibet分类
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向明学,金鹏杰,段双全,许鹏辉,拉多..西藏圆齿红景天茎内曲霉属真菌系统发育分析[J].安徽农业科学,2018,46(5):117-119,3.
