安徽农业科学2018，Vol.46Issue(11)：1-3,9,4.
人参蛋白质不同提取方法及含量的比较
Comparision on Different Extraction Methods and Contents of Panax ginseng Protein
摘要
Abstract
[Objective]The best method of extraction of ginseng protein was optimized,and the effect of different growth years on protein con-tent was compared.[Method]The extraction method of five kinds of ginseng protein was studied by acetone precipitation method,polyethylene glycol 6000 precipitation method,polyethylene glycol 20000 precipitation method,phenol extraction method and Trizol extraction method,and different growth years of ginseng protein.The protein content was determined by Bradford method,SDS-PAGE identification of proteins.[Re-sult]The purity of the protein was the highest in the acetone extraction method, and the electrophoretic bands were the most clear and com-plete, and the content of ginseng protein increased with the growth year.[Conclusion]Acetone precipitation method was a better method for the extraction of ginseng proteins.The protein content of ginseng was the highest in 5 years.关键词
人参蛋白/提取方法/SDS-PAGE电泳Key words
Ginseng protein/Extraction method/SDS-PAGE electrophoresis分类
农业科技引用本文复制引用
任雨贺,刘淑莹,万茜淋..人参蛋白质不同提取方法及含量的比较[J].安徽农业科学,2018,46(11):1-3,9,4.基金项目
吉林省中医药科技项目(2017015) （2017015）
吉林省科技发展计划项目(201603003YY) （201603003YY）
国家自然科学基金面上项目(21475012). （21475012）
