安徽农业科学2018，Vol.46Issue(11)：81-83,3.
珍稀濒危植物银缕梅ISSR-PCR反应体系建立及优化
Establishment and Optimization of ISSR-PCR Reaction System for Rare and Endangered Plant Parrotia subaequalis
摘要
Abstract
[Objective]To establish and optimize ISSR-PCR reaction system.[Method]Effect for ISSR-PCR reaction of Parrotia subaequalis on 5 factors(Mg2 +,dNTPs,DNA template,Taq polymerase and primer)were analyzed by orthogonal design and single factor test.[Result]The optimal ISSR-PCR reaction system(25 μL)mixture contained DNA template(20 ng/μL)2.50 μL,primer(10 μmol/L)1.00 μL,Taq polymerase (5 U/μL)0.10 μL,Mg2 +(25 mmol/L)3.00 μL,dNTPs(2.5 mmol/L)1.50 μL.[Conclusion]The establishment of ISSR-PCR reaction system for P.subaequalis laid the foundation for the further use of ISSR to study molecular marker-assisted breeding,molecular identity and genetic diver-sity.关键词
银缕梅/ISSR/PCR反应体系/资源保护Key words
Parrotia subaequalis/ISSR/PCR reaction system/Resources protection分类
农业科技引用本文复制引用
陈云霞,路志远,肖卓恒..珍稀濒危植物银缕梅ISSR-PCR反应体系建立及优化[J].安徽农业科学,2018,46(11):81-83,3.基金项目
国家级大学生创新训练项目(201612213012). （201612213012）
