长春中医药大学学报2018，Vol.34Issue(2)：224-227,4.DOI:10.13463/j.cnki.cczyy.2018.02.007
痛泻安肠方对腹泻型肠易激综合征模型大鼠结肠TRPV1表达及血浆SP、CGRP含量的影响
Effect of Tongxie Anchang decoction on the expression of TRPV1 and the SP and CGRP of blood plasma in the colon of rats with diarrhea predominant irritable bowel syndrome
摘要
Abstract
Objective To investigate the effect of Tongxie Anchang decoction on the expression of TRPV1 and the content of serum SP and CGRP in rats with diarrhea predominant irritable bowel syndrome. Methods The model of IBS-D was made by intracolonic instillation of acetic acid, balloon rectal dilatation and tail clamping stimulation. After the modeling, 60 SD rats were randomly divided into blank group, model group, intervention group (low-dosage, middle-dosage, high-dosage) and control group. Rats were observed before and after treatment of Abdominal withdrawal reflex (AWR) score and Bristol score; Western Blot was used to detect the expression of TRPV1 in colon, and the expressions of TRPV1mRNA in colon were detected by Real-Time and PCR; The expression of serum substance P(SP) and calcitonin gene related peptide (CGRP) were tested by Elisa. Results Compared with control group, the rats in the model group of visceral sensitivity threshold decreased (P<0.05, P<0.01), fecal Bristol score was significantly increased (P<0.01); After treatment, The visceral sensitivity threshold of rats in low, medium and high dose groups and Dicetel group was increased (P<0.05, P<0.01), fecal Bristol grading score was decrease (P<0.01), colon TRPV1 protein expression decreased (P<0.05, P<0.01), TRPV1mRNA expression decreased (P<0.01),serum levels of SP and CGRP were decreased(P<0.05,P<0.01).Conclusion Tongxie Anchang decoction can reduce visceral hypersensitivity of IBS-D rats, the mechanism may be achieved by downregulating colon TRPV1 protein expression, decreasing colon TRPV1 mRNA expression, decreasing the Serum SP and CGRP levels, and upregulating visceral sensitivity.关键词
肠易激综合征/痛泻安肠方/降钙素基因相关肽/P物质/辣椒素受体Key words
IBS/Tongxie Anchang decoction/Calcitonin gene related peptide/substance P/TRPV分类
医药卫生引用本文复制引用
韩亚飞,王允亮,郭一,石磊,贾博宜,李军祥..痛泻安肠方对腹泻型肠易激综合征模型大鼠结肠TRPV1表达及血浆SP、CGRP含量的影响[J].长春中医药大学学报,2018,34(2):224-227,4.基金项目
国家科技支撑计划项目"病证结合治疗腹泻型肠易激综合征的临床示范性研究"(2013BAI02B05). （2013BAI02B05）
