大豆科学2018,Vol.37Issue(2):215-223,9.DOI:10.11861/j.issn.1000-9841.2018.02.0215
大豆芽期和苗期耐盐性评价指标筛选
Related Indices Selection of Soybean Salt Tolerance at Germination and Seedling Stages
摘要
Abstract
Soybean salt tolerance is one of the key factors in improving soybean yield in saline soils.In this study,four cultivars of soybean(Hedou 3,Xudou 14,Zhonghuang 55,Lindou 10) were treated with different concentrations of saline solution at the germination stage(50,75 and 100 mmol·L-1) and the seedling stage (100,200 and 250 mmol·L-1).We examined some morphological and physiological indicators at three concentration levels in two stages and transfered these measurement indicators to the subjection values of salt tolerance index and the sum subjection value.Then we studied the salt tolerance of the 4 cultivars by comparing the differences of salt toxicity coefficients of subjection values at the stage of germination and seedling respectively.Finally,the cluster analysis of salt toxicity coefficients of multi-indices was carried out.The results showed that the subjection values of salt tolerance index of root length,hypocotyl length,dhy weight of root,water content,fresh biomass,fresh weight of root and vigor index were significantly correlated with SUM subjection values at germination stage.And the subjection values of salt tolerance index of MDA content,plant height,dry weight of root and fresh weight of root were significantly correlated with SUM subjection values at seedling stage.Clustering analysis showed different salt tolerance among different cultivars and growth stages.No similarity soybean varieties were clustered at germination stage using the eight subjection values of salt tolerance index among the four soybean varieties,which indicated similar level of salt tolerance.At seedling stage,the cluster analysis grouped the varieties into three clusters.The first group of salt-resistant soybean varieties were Xudou 14 and Lindou 10,the second was moderately salt-tolerant cultivar Zhonghuang 55 and the third group was salt-sensitive cultivar Hedou 3.In selection of salt tolerance index,hypocotyl length,dry weight of root,water content,fresh biomass,fresh weight of root and root length could be served as the indicators to assess the salt tolerance of the cultivars at germination stage,while plant height and MDA content could be used to evaluate the salt tolerance at seedling stage.关键词
大豆/芽期/苗期/耐盐性/耐盐指数/隶属函数Key words
Soybean/Germination stage/Seedling stage/Salt tolerance/Salt tolerance index/Subjection values分类
农业科技引用本文复制引用
牛远,杨修艳,戴存凤,王博文,任高磊,吴静磊,王飞兵,陈新红..大豆芽期和苗期耐盐性评价指标筛选[J].大豆科学,2018,37(2):215-223,9.基金项目
江苏省高等学校自然科学研究项目(17KJB210001) (17KJB210001)
淮安市科技指导性项目(Z413B16352) (Z413B16352)
大学生创新创业训练计划(Z205C17689). (Z205C17689)