大豆科学2018，Vol.37Issue(2)：303-309,7.DOI:10.11861/j.issn.1000-9841.2018.02.0303
大豆异黄酮的提取优化与测定
Optimization of the Extraction and Determination of Soybean Isoflavones
摘要
Abstract
In this experiment,the extraction method of soybean isoflavones was optimized,and a high performance liquid chromatography (HPLC) standard measurement system for the determination of isoflavones for laboratory extraction were established.The extraction conditions were as follows:Skimmed soy sample powder added 10 mL of chromatographic grade methanol at a volume of 80％,and placed at room temperature for 2 h.After the installation of ultrasonic power 200 W,80℃ water bath for 12 h,centrifuged at 12 000 r· min-1 for 15 min,taken the supernatant by 0.45 μm microporous membrane filtration,and to be stored at 4℃.The optimal HPLC method was as follows:The mobile phase was methanol-water (volume ratio 30∶70),column temperature set at 40℃,the wave length was 254 nm,the flow rate was 1 mL· min-1,the sample volume was 10 μL,set injection time for 26 min,and calculated by peak area method.The results showed that the optimized HPLC method was efficient and accurate in extracting soybean isoflavones.It could provide technical reference for efficient extraction of soy isoflavones in laboratory.关键词
大豆提取物/大豆异黄酮/高效液相色谱法(HPLC)Key words
Extract of soybean/Soy isoflavones/High performance liquid chromatography (HPLC)分类
农业科技引用本文复制引用
李雪莹,王铎,范素杰,韩笑,姚丹,张大瑜,卢敏,张君..大豆异黄酮的提取优化与测定[J].大豆科学,2018,37(2):303-309,7.基金项目
吉林省科技发展计划项目(20150204004NY). （20150204004NY）
