动物医学进展2018，Vol.39Issue(3)：1-4,4.
猪流行性腹泻病毒经典株与变异株高分辨率熔解曲线方法的建立
Development of PCR-HRM Method for Identifying PEDV Mutant Strains and Classic Strains
摘要
Abstract
In order to build a differential diagnosis of high resolution melting curve method of porcine epi-demic diarrhea virus classic and mutant strains,based on the differences in S gene sequence,a pair of prim-ers were designed for RT-PCR amplification,and then high resolution melting-curve(HRM)was estab-lished for differentiating between mutant strains and classic strains.10 clinical samples were screened by this assay,and 4 positive for classical strains and 6 positive for mutant strains were found.The experiments revealed that the developed RT-PCR and HRM assay was specific and sensitive,and it could be used for rapid and accurate differentiation of PEDV mutant strains and classic strains.关键词
猪流行性腹泻病毒/经典株/变异株/高分辨率熔解曲线Key words
Porcine epidemic diarrhea virus/mutant strain/classic strain/high resolution melting-curve分类
农业科技引用本文复制引用
刘向楠,陈梅丽,饶丹,丛锋,练月晓,曾繁文,黄韧,张钰,郭鹏举,罗满林..猪流行性腹泻病毒经典株与变异株高分辨率熔解曲线方法的建立[J].动物医学进展,2018,39(3):1-4,4.基金项目
广东省科技计划项目(2015B070701003) （2015B070701003）
广州市科技计划项目(201707010440) （201707010440）
