动物医学进展2018，Vol.39Issue(3)：19-23,5.
四川地区猪细环病毒1型的流行病学调查和分子特征研究
Molecular Epidemiology and Molecular Characterization of TTSuV1 in Sichuan Province
摘要
Abstract
The obj ective of this study was to investigate the prevalence of porcine torque teno sus virus 1 (TTSuV1)in Sichuan province and to better study the genetic variation of TTSuV.In this study,a total of 140 clinical health pig sera were collected in 5 different intensive pig farms of Sichuan province.The preva-lence of TTSuV1 was detected by PCR.The genomes of TTSuV1 a and TTSuV1 b were amplified according to the complete gene sequence of TTSuV1 published by NCBI.The results demonstrated that the TTSuV1 a and TTSuV1b infection rates were 49.29% (95%CI:40.7%-57.9%)and 39.29% (95%CI:31.1%-47.9%), respectively.The near-full genome length of TTSuV1a was 2 497 bp and 2 693 bp of TTSuV1b by cloning and sequencing.The nucleotide identities of ORF1 and ORF2 sequences of TTSuV1 a were 7 9.3 %-9 7.5 % and 92.7%-98.2% to other strains in GenBank,respectively.The nucleotide identities of the ORF1 and ORF2 sequence of TTSuV1b were 100% and 97.1%-98.6%,with those of reference strains.The results may provide basic data on infection and prevalence of TTSuV1 virus in swine herds in Sichuan province, and provide a theoretical basis for further study of the genetic evolution of TTSuV virus.关键词
猪细环病毒1型/分子流行病学/全基因组/进化分析Key words
Porcine torque teno sus virus 1/molecular epidemiology/complete gene sequence/evolutionary analysis分类
农业科技引用本文复制引用
阮文强,宝志鹏,覃思楠,陈新诺,何欢,岳华,张斌..四川地区猪细环病毒1型的流行病学调查和分子特征研究[J].动物医学进展,2018,39(3):19-23,5.基金项目
"十三五"国家重点研发计划项目(2016YFD0500705) （2016YFD0500705）
西南民族大学研究生创新科研项目(CX2017SZ054) （CX2017SZ054）
