动物医学进展2018，Vol.39Issue(3)：124-128,5.
我国部分地区奶牛乳房炎主要病原菌的分离鉴定及药敏试验
Isolation,Identification and Drug Susceptibility Test of Main Pathogens of Dairy Cow Mastitis in Some Regions of China
摘要
Abstract
In order to understand the epidemic situation and drug susceptibility of mastitis pathogens in dair-y cows in China,247 batches of milk samples of dairy cows with mastitis were collected for bacterial isola-tion and drug susceptibility test in 5 provinces and autonomous regions of Inner Mongolia,Heilongjiang, Shandong,Shanghai and Hebei provinces.The results showed that 175 strains of bacteria were isolated from 247 milk samples,Escherichia coli 65 strains,accounting for 37.14%;Staphylococcus aureus 30 strains, accounting for 17.14%;Enterococcus faecalis 21 strains,accounting for 12.00%;Klebsiella 18 strains, 10.29%;Streptococcus 13 strains,accounting for 7.43%;Citrobacter freudii 12 strains,accounting for 6.86%;Staphylococcus saprophyticus 5 strains,accounting for 2.86%;Proteus 4 strains,accounting for 2.2 9 %;Serratia marcescens 3 strains,accounting for 1.7 1 %;Staphylococcus epidermidis 2 strains,ac-counting for 1.14%;Staphylococcus chromogenes 1 strain,accounting for 0.57% and Buttiauxella agrestis 1 strain,accounting for 0.57%.Drug resistance test results showed that the isolation of Gram-positive bac-teria on the highest rate of resistance to aztreonam,reached 100%,followed by penicillin(80.56%),ofloxa-cin (43.06%),cefoxitin (43.06%)and meropenem (41.67%)were higher than 40% resistance.Resistance rates were less than 10% for vancomycin (all sensitive),oxacillin (all sensitive),linezolid (all sensitive), ogomintine (all sensitive),cotrimoxazole (2.78%),ciprofloxacin (6.94%)and ampicillin (9.72%).The re-sistance of Gram-negative bacteria to ampicillin was the highest,reaching 83.50%,followed by florfenicol 44.66% and tetracycline 43.69% higher than 40%.Resistance rates were less than 10% with meropenem (all sensitive),cefoxitin (5.83%)and aztreonam (9.71%).The results provided a reference for the preven-tion and control of dairy cow mastitis and clinical medication.关键词
奶牛乳房炎/病原菌/分离鉴定/耐药性Key words
dairy cow mastitis/pathogen/isolation and identification/drug resistance分类
农业科技引用本文复制引用
杜琳,王丽芳,冯小慧,张三粉,宋洁,姚一萍,史培,申捷..我国部分地区奶牛乳房炎主要病原菌的分离鉴定及药敏试验[J].动物医学进展,2018,39(3):124-128,5.基金项目
国家农产品质量安全风险评估重大专项(GJFP201700803) （GJFP201700803）
内蒙古农牧业创新基金项目(2017CXJJM09) （2017CXJJM09）
