动物营养学报2018，Vol.30Issue(4)：1522-1529,8.DOI:10.3969/j.issn.1006-267x.2018.04.037
饲料中添加低聚木糖对凡纳滨对虾幼虾消化酶活力、肠道形态及细菌数量的影响
Effects of Dietary Xylo?Oligosaccharides on Digestive Enzymes Activities, Intestinal Morphology and Bacteria Numbers of Juvenile Litopenaeus vannamei
摘要
Abstract
This experiment was conducted to investigate the effects of dietary xylo?oligosaccharides(XOS)on digestive enzyme activities, intestinal morphology and bacteria numbers of juvenile Litopenaeus vannamei. A total of 800 juvenile Litopenaeus vannamei with an average body weight of(0.67±0.02)g were randomly as?signed to 4 groups with 4 replicates per group and 50 shrimps per replicate. Shrimps in the control group were fed a basal diet, and others in the experimental groups were fed the basal diets supplemented with 200, 400, and 600 mg/kg XOS, respectively. The experiment lasted for 42 days. The results showed as follows: 1) compared with the control group,the intestinal amylase activity and hepatopancreas protease activity of shrimps in experimental groups were significantly increased(P<0.05),the intestinal and hepatopancreas protease activ?ity in 200 mg/kg group, the intestinal and hepatopancreas lipase activity in 400 mg/kg group, and stomach protease activity in 400 mg/kg group were significantly increased(P<0.05). 2)Compared with the control group, the hepatopancreas total protein content, intestinal wall thickness and intestinal villus height of shrimps in 400 mg/kg group were significantly increased(P<0.05). 3)Compared with the control group, the intesti?nal total bacteria and Bifidobacteria numbers of shrimps in experimental groups were significantly increased (P<0.05), while the intestinal vibrio number was significantly decreased(P<0.05). In conclusion, dietary XOS can improve digestive enzyme activities and intestinal morphology, increase intestinal total bacteria and Bifidobacteria numbers, decrease intestinal Vibria number of juvenile Litopenaeus vanname.关键词
低聚木糖/凡纳滨对虾/消化酶活力/肠道形态/肠道细菌Key words
xylo⁃oligosaccharides/Litopenaeus vannamei/digestive enzyme activities/intestinal morphology/intestinal bacteria分类
农业科技引用本文复制引用
陈晓瑛,王国霞,孙育平,陈冰,莫文艳,赵红霞,曹俊明,黄燕华..饲料中添加低聚木糖对凡纳滨对虾幼虾消化酶活力、肠道形态及细菌数量的影响[J].动物营养学报,2018,30(4):1522-1529,8.基金项目
广东省科技厅省重点实验室项目(2014B030301054) （2014B030301054）
广东省农业科学院学科团队建设项目(水产营养与饲料学科团队) （水产营养与饲料学科团队）
广东省级现代农业产业技术推广体系建设项目(2017LM1082,2017LM1083) （2017LM1082,2017LM1083）
