动物营养学报2018，Vol.30Issue(4)：1538-1546,9.DOI:10.3969/j.issn.1006-267x.2018.04.039
壳寡糖对小鼠生长性能、血清免疫和抗氧化指标的影响
Effects of Chitosan Oligosaccharide on Growth Performance, Serum Immune and Antioxidant Indexes of Mice
摘要
Abstract
This experiment was carried out to investigate the effects of gavage administration of different doses of chitosan oligosaccharides(COS)on growth performance, serum immunoglobulins contents, cytokines lev?els and antioxidant enzyme activities of mice. Sixty 5?week?old specific pathogen?free(SPF)ICR male mice were selected and randomly divided into 6 groups,including 1 control group and 5 experimental groups with 10 mice each. The mice in 5 experimental groups were given gavage administration of COS(dissolved in normal saline)at levels of 0.2,0.4,0.6,0.8 and 1.0 g/kg BW,and the mice in control group were given gavage ad?ministration of the same volume of normal saline. The experiment lasted for 6 weeks. The results showed as fol?lows:1)gavage administration of different doses of COS had no significant influence on body weight of mice (P>0.05). 2)Thymus index was significantly increased by gavage administration of 0.6 g/kg BW COS,and it was increased by 32.87% compared with the control group(P<0.05). 3)Gavage administration of 0.6 g/kg BW COS significantly increased the contents of serum immunoglobulin A(IgA)and immunoglobu?lin(IgM), and they were increased by 12.14% and 20.69%, respectively, compared with the control group (P<0.05). 4)No significant differences were found in serum interleukin?6(IL?6), interleukin?10(IL?10), interleukin?1β(IL?1β), transforming growth factor?β(TGF?β)and tumour necrosis factor?α(TNF?α)by gavage administration of different doses of COS(P>0.05). 5)The activity of myeloperoxidase(MPO)was significantly increased by gavage administration of 0.6 g/kg BW COS, and it was increased by 27.61% com?pared with the control group(P<0.05). The activity of peroxidase(POD)was significantly increased by ga?vage administration of 0.8 g/kg BW COS, and it was increased by 45.08% compared with the control group (P<0.05). In summary, under the conditions of this experiment,COS has function of promoting the develop?ment of immune organs and regulating serum immune and antioxidant indexes of mice.关键词
壳寡糖/ICR小鼠/生长性能/免疫球蛋白/细胞因子/抗氧化酶Key words
chitosan oligosaccharides/ICR mice/growth performance/immunoglobulin/cytokine/antioxi⁃dant enzyme分类
农业科技引用本文复制引用
张南南,李兰兰,张晓昀,闻正顺,邹晓庭..壳寡糖对小鼠生长性能、血清免疫和抗氧化指标的影响[J].动物营养学报,2018,30(4):1538-1546,9.基金项目
浙江省自然科学基金(LY17C170001,LQ13C200002) （LY17C170001,LQ13C200002）
