动物营养学报2018，Vol.30Issue(4)：1574-1581,8.DOI:10.3969/j.issn.1006-267x.2018.04.043
不同氮能比饲粮对妊娠环江香猪羊水和尿囊液生化参数的影响
Effects of Different Nitrogen/Energy Diets on Biochemical Parameters in Amniotic Fluid and Allantoic Fluid of Pregnant Huanjiang Mini?Pigs
摘要
Abstract
The present study was conducted to investigate the effects of different nitrogen/energy diets on bio?chemical parameters in amniotic fluid and allantoic fluid of pregnant Huanjiang mini?pigs. Forty?eight primipa?rous Huanjiang mini?pigs were randomly assigned into one of two groups and reared in 8 pens(replicates) with 3 pigs per pen. After service,the animals in the two groups were fed high nitrogen/energy diet(digestible energy was 14.73 MJ/kg, crude protein content was 13.11%, and nitrogen/energy was 0.89)and low nitro?gen/energy diet(digestible energy was 12.24 MJ/kg, crude protein content was 9.77%, and nitrogen/energy was 0.80), respectively. At 45, 75, and 110 days of pregnancy, one pig was selected randomly from each pen and sacrificed. The amniotic fluid and allantoic fluid of fetuses with maximal body weight(BW), middle BW and minimal BW per litter were collected for determining biochemical parameters. The results showed that under the same pregnant time and standard body weight conditions, compared with feeding the low nitrogen/energy diet, feeding the high nitrogen/energy diet significantly increased the alkaline phosphatase(ALP)ac?tivity in allantoic fluid of fetuses with minimal BW at 75 days of pregnancy(P<0.05), while significantly de?creased the aspartate aminotransferase(AST)activity in allantoic fluid of fetuses with middle or minimal BW at 75 days of pregnancy and fetuses with minimal BW at 110 days of pregnancy, the uric acid(UA)content of fetuses with maximal BW at 45 and 75 days of pregnancy,the creatine kinase(CK)activity of fetuses with middle or minimal BW at 45 days of pregnancy and fetuses with maximal BW at 75 days of pregnancy(P<0.05). Compared with the fetuses with minimal BW, the albumin(ALB)content in amniotic fluid of fetuses with maximal BW at 45 and 75 days of pregnancy in high nitrogen/energy diet group was significantly in?creased(P<0.05), as well as at 45 and 110 days of pregnancy in low nitrogen/energy diet group(P<0.05). With the pregnant time extension, the urea(UREA)and UA contents in amniotic fluid and UA content in al?lantoic fluid of fetuses in two diet groups were gradually increased. These findings suggested that the high nitro?gen/energy diet can promote the growth and development of fetuses by regulating the biochemical parameters in amniotic fluid and allantoic fluid;the fetuses with maximal BW presents higher utilization efficiency of protein than the fetuses with middle or minimal BW.关键词
环江香猪/妊娠时间/饲粮氮能比/羊水/尿囊液/生化参数Key words
Huanjiang mini⁃pigs/pregnant time/dietary nitrogen/energy/amniotic fluid/allantoic fluid/bi⁃ochemical parameters分类
农业科技引用本文复制引用
赵越,孔祥峰,姬玉娇,耿梅梅,李华伟,王占彬..不同氮能比饲粮对妊娠环江香猪羊水和尿囊液生化参数的影响[J].动物营养学报,2018,30(4):1574-1581,8.基金项目
国家自然科学基金面上项目(31572421,31270044) （31572421,31270044）
中科院"西部之光"人才培养计划重点项目 （）
