高等学校化学学报Issue(4)：667-673,7.DOI:10.7503/cjcu20170459
RRLC-Q-TOF-MS研究人参二醇型皂苷Rb1,Rb2和Rc的化学转化
Chemical Transformation of Protopanaxadiol Type Ginsenoside Rb1, Rb2and Rc Analyzed by RRLC-Q-TOF-MS
摘要
Abstract
The developed rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry(RRLC-Q-TOF-MS)method was applied for analyzing the chemical transformation of protopanaxadiol(PPD)type ginsenoside Rb1, Rb2and Rc under acidic condition. And Rb1, Rb2, Rc and conversion products were analyzed in the ginseng processing. The experimental results demonstrated that chemical transformation mechanisms of protopanaxadiol type ginsenoside in acidic condition included saccharide hydrolysis,Δ20(21)-or Δ20(22)-dehydration,and hydration addition reactions at C24 and C25. The RRLC-Q-TOF-MS/MS analysis included the specific product ions of aglycone PPD(m/z 459),Δ20(21)-or Δ20(22)-dehydration-PPD(m/z 441), and C24, C25 hydrated-PPD(m/z 477). The tandem mass spectrometry(MS/MS)was discussed to provide a basis for structural characterization. The chemical trans-formation pathway for protopanaxadiol type ginsenoside was summarized. The established method was applied for analyzing PPD ginsenosides changes in raw white ginseng and processed red ginseng(100 and 120 ℃).关键词
人参二醇型皂苷/高分离快速液相色谱-四极杆-飞行时间质谱/化学转化Key words
Protopanaxadiol type ginsenoside/Rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry(RRLC-Q-TOF-MS)/Chemical transformation分类
化学化工引用本文复制引用
赵乐凤,焦传新,李慧,焦丽丽,马悦,吴巍,刘淑莹..RRLC-Q-TOF-MS研究人参二醇型皂苷Rb1,Rb2和Rc的化学转化[J].高等学校化学学报,2018,(4):667-673,7.基金项目
吉林省科技发展重大项目(批准号:20170204010YY)和人参化学与药理重点实验室探索项目(批准号:20160101334JC)资助.Supported by the Science and Technology Development Plan Project of Jilin Province,China(No.20170204010YY)and the Related Project of Key Lab of Ginseng Chemistry and Pharmacology of Jilin Province,China(No.20160101334JC). （批准号:20170204010YY）
