高等学校化学学报Issue(4)：807-816,10.DOI:10.7503/cjcu20170542
电纺纤维的刚度对iPS-MSCs黏附和迁移的影响
Effects of Electrospun Fiber-Stiffness on Adhesion and Migration of iPS-MSCs
摘要
Abstract
The stiffness of as-electrospun poly-L-lactic acid(PLLA)fibers was altered by annealing treatment, and then influence of fiber stiffness on the morphology, proliferation and migration of induced pluripotent stem cell-derived mesenchymal stem cells(iPS-MSCs)were evaluated. The results showed that annealing treatment had negligible influence on the diameter of PLLA fibers[before:(1.26±0.25)μm and after:(1.24 ± 0.26)μm]. However, fiber crystallinity and mechanical properties were enhanced, which resulted in 1.73 times stiffer than that of fibers before annealing treatment. The cell spreading area and proliferation of iPS-MSCs cultured on the treated PLLA scaffold after 1 and 7 d of incubation were 1.78 and 1.18 times higher,respectively, than that of untreated scaffold. The increase of fiber stiffness can affect the migration of iPS-MSCs and promote up-regulated expression of the migration-related genes Integrinβ1, RhoA and Rock1. Collectively,this study demonstrated that increasing fiber stiffness by annealing may be used as an important modality to regulate cell biological functions of the cells in engineering tissues.关键词
电纺纤维/纤维刚度/诱导多能干细胞来源的间充质干细胞/细胞黏附/细胞迁移Key words
Electrospun fiber/Fiber stiffness/Induced pluripotent stem cell-derived mesenchymal stem cells (iPS-MSCs)/Cell adhesion/Cell migration分类
化学化工引用本文复制引用
余哲泡,袁卉华,易兵成,王先流,张赵文斌,张彦中..电纺纤维的刚度对iPS-MSCs黏附和迁移的影响[J].高等学校化学学报,2018,(4):807-816,10.基金项目
国家重点研发计划项目(批准号:2016YFC1100203)、国家自然科学基金(批准号:31570969,31771050)和上海市科委基础研究重点项目(批准号:14JC1490100)资助.Supported by the National Key Research and Development Program of China(No.2016YFC1100203),the National Natural Science Foundation of China(Nos.31570969,31771050)and the Key Project of Science and Technology of Shanghai Municipality,China(No.14JC1490100). （批准号:2016YFC1100203）
