医学分子生物学杂志2018，Vol.15Issue(1)：30-34,5.DOI:10.3870/j.issn.1672-8009.2018.01.006
野黄芩苷对苯并芘致人胚肺成纤维细胞DNA损伤的保护作用
Protective Effect of Scutellarin on DNA Damage Induced by Benzopy-rene in Human Embryonic Lung Fibroblasts
摘要
Abstract
Objective To study the protective effect of scutellarin on DNA damage induced by benzo[a]pyrene(B(a)P). Methods ① The p53 -/ -MEF cells(p53-GFP cells)were successfully transfected with p53-GFP plasmid to screen the activation of p53 promoter induced by small molecule compounds such as scutellarin.②The MRC-5 cells were divided into DMSO control group,B(a)P treatment group,B(a)P + scutellarin group; p-p53(Ser15)and γH2AX pro-tein expression levels were detected by Western blotting.③The MRC-5 cells were divided into DM-SO control group,B(a)P treatment group, B(a)P + scutellarin group; Western blotting was used to detect the changes of apoptosis-related protein PARP in MRC-5 cells.Results Fluorescence Results showed that 10 μmol/L of B(a)P significantly activated p53-GFP fluorescence, while the fluorescence activation was significantly inhibited by scutellarin + B(a)P.Western blotting showed that in comparison with DMSO group, the expression of p-p53(Ser15)and γH2AX in MRC-5 cells in B(a)P group was increased, PARP cleavage enhanced, whereas p-p53 and γH2AX were significantly lower than those in the B(a)P + scutellarin group, at the same time, the cleavage of PARP was significantly decreased in a concentration-dependent manner. Conclu-sion In this study,by stable transfection with p53-GFP plasmid in p53-/-MEF cells,it was ini-tially observed that scutellarin could protect against DNA damage induced by benzopyrene.Further studies in MRC-5 cells demonstrated that the combination of scutellarin and B(a)P can reduce the expression of p-p53(Ser15)and γH2AX in MRC-5 and decrease the expression of apoptosis-related proteins in MRC-5 cells,suggesting that scutellarin has a protective effect on DNA damage induced by benzopyrene in MRC-5 cells,and its mechanism may be related to inhibition of DNA damage and apoptosis pathway.关键词
野黄芩苷/苯并芘/人胚肺成纤维细胞/DNA损伤Key words
scutellarin/benzopyrene/human embryonic lung fibroblasts/DNA damage分类
医药卫生引用本文复制引用
高茜,刘丹,彭雷,刘静,罗瑛,夭建华,张继虹..野黄芩苷对苯并芘致人胚肺成纤维细胞DNA损伤的保护作用[J].医学分子生物学杂志,2018,15(1):30-34,5.基金项目
云南省烟草化学重点实验室开放基金(No.FW32104132-4) （No.FW32104132-4）
This work was supported by a grant from the Yunnan Tobacco Chemical Key Laboratory Open Fund Project(No.FW32104132-4) （No.FW32104132-4）
