医学分子生物学杂志2018，Vol.15Issue(2)：68-72,5.DOI:10.3870/j.issn.1672-8009.2018.02.002
Juxtanodin(JN)在少突胶质细胞分化中的表达及其功能的初步研究
Expression of Juxtanodin(JN)in the Differentiation of Oligoden-drocytes and Its Possible Roles
摘要
Abstract
Objective To cultivate the primary oligodendrocytes and examine JN expression at different stages of their differentiation and build eukaryotic expression recombinant plasmid contai-ning JN gene and explore its possible roles in the development of oligodendrocytes.Methods Oligo-dendrocytes were isolated and purified from cerebral cortex tissue of newborn rats and then the cells were identified against specific makers and JN by using Western blotting.The rat JN recombinant eu-karyotic expression plasmid was constructed by conventional method, including polymerase chain re-action(PCR), restriction enzyme digestion and ligation.The successfully reconstructed plasmid, as identified by sequence analysis, was tansfected into 293 T cells and detected by Western blottong.Meanwhile, the reconstructed plasmid was tansfected into OLN93 cells to observe the mor-phological changes of cells.Results Oligodendrocytes were cultivated successfully and JN was ex-pressed at the late stage of oligodendrocyte differentiation.Our results also showed that the JN recom-binant eukaryotic expression plasmid was successfully and constructed and JN could promote the for-mation of oligodendrocyte processes.Conclusion JN appeared at the late stage of oligodendrocyte development and it could promote the formation of oligodendrocyte processes and, possible, the for-mation of myelin sheath.关键词
少突胶质细胞/JN/OLN93细胞系Key words
oligodendrocyte/JN/OLN93 cells分类
医药卫生引用本文复制引用
王涛,曹冰清,薛延莉,韩征,何堪生..Juxtanodin(JN)在少突胶质细胞分化中的表达及其功能的初步研究[J].医学分子生物学杂志,2018,15(2):68-72,5.基金项目
国家自然科学基金(No.31100787),陕西省自然科学基础研究计划项目(No.2017 JM8029),陕西省社会发展科技攻关项目(No.2014 K11-03-02-07)This work was supported by grants from the National Natural Science Foundation of China(No.31100787), Natural Sciences Foundation of Shaanxi Provincial of China(2017 JM8029)and Scientific And Techno-logical Project of Shaanxi Provincial of China(No.2014 K11-03-02-07) （No.31100787）
