广西林业科学2018,Vol.47Issue(1):13-17,5.
基于SLAF-seq技术的油茶SNP位点开发及杂交后代早期鉴定
Development of SNP Markers of Camellia oleifera Based on SLAF-seq and Early Identification of Hybrids
摘要
Abstract
In order to develop a large number of molecular markers for early identification of Camellia oleifera hybrids,9 samples of Camellia oleifera(including 5 parents and 4 progenies)were sequenced by SLAF-seq technology and SNP markers were developed.Finally,a total of 1 644 616 population SNPs were developed based on the polymorphic SLAFs. Paternity analysis of the 9 samples of C.oleifera based on the population SNPs revealed that ZJ11,ZJ14 and ZJ24 was hybrid progenies of Cenruan 2 and Cenruan 11,CR-11 and CR-22,CR-11 and CR-24,respectively,while You 31 was a hybrid progeny of CR-11 and CR-23.关键词
油茶/SLAF-seq/分子标记/SNPKey words
Camellia oleifera/SLAF-seq/molecular marker/SNP分类
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刘凯,王东雪,江泽鹏,莫健芳,曾雯珺,张乃燕,梁国校..基于SLAF-seq技术的油茶SNP位点开发及杂交后代早期鉴定[J].广西林业科学,2018,47(1):13-17,5.基金项目
广西自然科学基金项目(2015GXNSFAA139075 ()
2017JJ130004) ()
广西特色经济林培育与利用重点实验室项目(16-A-01-01) (16-A-01-01)