南方农业学报2018，Vol.49Issue(3)：462-468,7.DOI:10.3969/j.issn.2095-1191.2018.03.08
不同氮素来源对烤烟上部叶碳氮代谢及其基因表达的影响
Influence of different nitrogen sources on carbon and nitrogen metabolism and gene expression in tobacco upper leaves
摘要
Abstract
[Objective]Effects of different nitrogen sources(organic nitrogen fertilizer and inorganic nitrogen fertili-zer)on carbon and nitrogen metabolism and key gene expression in the upper leaves of flue-cured tobacco were investiga-ted to provide theoretical basis for further regulating the application of fertilizer and improving the quality of tobacco leaves.[Method]Organic nitrogen fertilization and inorganic nitrogen fertilization were set as two treatments on the basis of the same nitrogen amount.The tobacco seedlings were sampled 60,70,80 and 90 d after transplanting.The ultrastruc-ture of the cells in different treatments were observed,and contents of total sugar,total nitrogen and nicotine in tobacco leaves were determined,then expression of key genes affecting carbon and nitrogen metabolism were analyzed.[Result]The starch granules treated by organic nitrogen fertilizer increased in the tobaccoupper leaf cells,the content and volume of osmiophilic granules also increased with the prolongation of transplanting time.Total sugar content in the two nitrogen fertilizer treatments decreased first and then increased in the upper leaves at mature period,and the one treated with or-ganic nitrogen fertilizer was higher than the other. The total nitrogen content in the two treatments decreased with the prolongation of growth period. Compared with the inorganic nitrogen fertilizer treatment,total nitrogen content in the treatment of organic nitrogen fertilizer was significantly lower(P<0.05,the same below)at the mature period.However, the total nitrogen contents in the two treatments tended to be consistent 90 d after transplanting.The nicotine content in the two nitrogen treatments showed an upward trend at mature period.Compared with the inorganic nitrogen fertilizer treat-ment,the nicotine content treated by organic nitrogen fertilizer was significantly lower 80 d after transplanting,but the nicotine content at other sampling periods were all higher than the other treatment.The analysis for key gene expression in carbon metabolism pathway showed that sucrose synthase gene(SuS1),starch synthase gene(SS1),granule bound starch synthase gene(GBSSI),amylopectin synthase gene(SBE1)and alpha amylase gene(AMML)were highly expressed,and this was the main reason for the large amount of accumulation of total sugar in tobacco upper leaves.The analysis for key gene expression in nitrogen metabolism pathway showed that the high expression level of nitrate transporter gene(NRT1) and ornithine decarboxylase gene(ODC)under organic nitrogen source was the main reason for the increase of nicotine content in upper leaves.[Conclusion]The application of organic nitrogen fertilizer is beneficial for the expression of key genes in carbon and nitrogen metabolism pathway of tobacco upper leaf at mature stage,which may increasethe content of total sugar and nicotine in tobacco upper leaf and decrease the total nitrogen content.关键词
烤烟/有机氮肥/无机氮肥/上部叶/碳氮代谢/基因表达Key words
tobacco/organic nitrogen fertilizer/inorganic nitrogen fertilizer/upper leaf/carbon and nitrogen me-tabolism/gene expression分类
农业科技引用本文复制引用
黄化刚,申燕,吴飞跃,杨振智,刘世碧,喻奇伟,贾宏昉..不同氮素来源对烤烟上部叶碳氮代谢及其基因表达的影响[J].南方农业学报,2018,49(3):462-468,7.基金项目
国家自然科学基金项目(31303817) （31303817）
河南省高等学校重点科研项目(15A210029) （15A210029）
贵州省烟草公司毕节市公司科技攻关项目(毕节合2014-59) （毕节合2014-59）
