南方农业学报2018，Vol.49Issue(3)：476-480,5.DOI:10.3969/j.issn.2095-1191.2018.03.10
甜瓜尾孢叶斑病病原分离与鉴定
Isolation and identification of pathogen causing Cercospora leaf spot of muskmelon
摘要
Abstract
[Objective]The pathogen causing leaf spot disease on muskmelon in greenhouse in Guangxi in recent years was clarified to provide reference for the effective control of the disease.[Method]The pathogenic fungi were isolated by single spore isolation method from leaves of diseased muskmelon.The pathogenicity of pathogenic fungi was verified by inoculation of detached leaves.Furthermore,the colony,conidiophores and conidia characteristics of the pathogen were observed.Homology analysis on rDNA-ITS gene sequences of the pathogenic fungi was conducted.[Result]The morpho-logical characteristics of the pathogen were consistent with Cercospora citrullina. And its rDNA-ITS gene sequences showed 99% similarity with C. citrullina. Based on the morphological and molecular characteristics,the pathogen was identified as C. citrullina.[Conclusion]The serious leaf spot appeared on muskmelon in greenhouse in Guangxi these years is caused by C.citrullina.It is a new disease on muskmelon in Guangxi.关键词
甜瓜/叶斑病/病原鉴定/瓜类尾孢Key words
muskmelon/leaf spot/identification of pathogen/Cercospora citrullina分类
农业科技引用本文复制引用
叶云峰,杜婵娟,覃斯华,柳唐镜,洪日新,付岗,李桂芬,黄金艳..甜瓜尾孢叶斑病病原分离与鉴定[J].南方农业学报,2018,49(3):476-480,5.基金项目
广西创新驱动发展专项资金项目(桂科AA17204097) （桂科AA17204097）
广西科学研究与技术开发计划项目(桂科能1598025-47) （桂科能1598025-47）
广西农业科学院科技发展基金项目(桂农科2017JM17) （桂农科2017JM17）
广西农业科学院基本科研业务专项项目(2015YT49) （2015YT49）
