南方农业学报2018，Vol.49Issue(3)：580-585,6.DOI:10.3969/j.issn.2095-1191.2018.03.25
原儿茶酸或绿原酸与抗菌药联用对鱼源链球菌的抑菌效果
Antibacterial effects of protocatechuic acid and chlorogenic acid combined with antibiotics against fish-source Streptococcus
摘要
Abstract
[Objective]The antibacterial effects of protocatechuic acid and chlorogenic acid combined with antibiotic on fish-source Streptococcus were studied to provide reference for the comprehensive prevention and treatment of fish-source Streptococcus disease.[Method]The minimum inhibitory concentrations(MIC)of protocatechuic acid,chlorogenic acid and antibiotics against fish-source Streptococcus were tested through double microdilution method,and fish-source Streptococcus were induced to subculture by inhibitory concentration(1/2MIC)of protocatechuic acid or chlorogenic acid combined with antibiotics respectively.Then the fractional inhibitory concentration indexes(FICI)of protocatechuic acid or chlorogenic acid combined with antibiotics respectively were determined by microdilution checkerboard method.[Re-sult]The MICs of protocatechuic acid and chlorogenic acid against fish-source Streptococcus were 2500 μg/mL and 6000 μg/mL.After fish-source Streptococcus being induced to subculture by 1250 μg/mL protocatechuic acid and 3000 μg/mL chlorogenic acid respectively combined with mequindox,amikacin,colistin,sulfamonomethoxine,clinafloxacin,and fosfomycin,the MICs of the antibiotics greattly decreased except sulfamonomethoxine. When protocatechuic acid was combined with the antibiotics mentioned above,the FICIs were 0.500,0.750,0.516,1.125,0.140 and 0.516 respective-ly.When chlorogenic acid was combined with the antibiotics mentioned above,the FICIs were 0.501,0.625,0.501,>2.000, 0.140 and 0.501.According to the criteria for FICI,there existed synergistic effects when protocatechuic acid was com-bined with mequindox and clinafloxacin;there existed additive effects when protocatechuic acid was combined with ami-kacin,colistin and fosfomycin,but there were indifferent effects when protocatechuic acid was mixed with sulfamono-methoxine.There were synergistic effects when chlorogenic acid was combined with amikacin and clinafloxacin;there existed additive effects after chlorogenic acid was mixed with mequindox,colistin and fosfomycin,but there were indif-ferent effects when chlorogenic acid was combined with sulfamonomethoxine.[Conclusion]The combination of protocate-chuic acid or chlorogenic acid with antibiotics can enhance the antibacterial activity of most tested antibiotics against fish-source Streptococcus and presents synergistic effects or additive effects.Therefore,it can be promoted in aquaculture.关键词
鱼源链球菌/原儿茶酸/绿原酸/联用/最小抑菌浓度(MIC)/部分抑菌浓度指数(FICI)Key words
fish-source Streptococcus/protocatechuic acid/chlorogenic acid/combination/minimum inhibitory concentration(MIC)/fractional inhibitory concentration indexes(FICI)分类
农业科技引用本文复制引用
柴贝贝,姜维佳,王莉贞,胡梅,赵雨川,吴永继,司红彬..原儿茶酸或绿原酸与抗菌药联用对鱼源链球菌的抑菌效果[J].南方农业学报,2018,49(3):580-585,6.基金项目
国家自然科学基金项目(31460675,31760746) （31460675,31760746）
广西重大科技攻关项目(桂科AA17204057) （桂科AA17204057）
南宁市科学研究与技术开发计划项目(20172135-3) （20172135-3）
