广西医科大学学报2018，Vol.35Issue(3)：281-285,5.DOI:10.16190/j.cnki.45-1211/r.2018.03.001
Acute andchronic cytotoxicity and genotoxicity induced by2,6-dichloro-1,4-benzoquinone in NIH3T3cells
Acute andchronic cytotoxicity and genotoxicity induced by 2,6-dichloro-1,4-benzoquinone in NIH3T3 cells
摘要
Abstract
Objective:This study aimed to evaluate the acute and chronic cytotoxicity and genotoxicity of 2,6-dichloro-1,4-benzoquinone (2,6-DCBQ).Methods:NIH3T3 cells were exposed to 2,6-DCBQ for 3 and 72 h,and relative cell viability was calculated.NIH3T3 cells were treated with different concentrations of 2,6-DCBQ for 24 h.The solvent and positive controls were dimethyl sulfoxide (DMSO) and 0.5 μmol/L ethylmethylsulfone,respectively.The values of Olive tail moment were measured by comet assay.NIH3T3 cells were then simultaneously treated with 5 μg/mL cytochalasin B and different concentrations of 2,6-DCBQ.The solvent and positive controls were DMSO and 1 μmol/L mitomycin C,respectively.Micronucleus rates were calculated after 48 h.Results:The half lethal doses of 2,6-DCBQ in NIH3T3 cells were 64.93 μmol/L for 3 h and 13.46 μmol/L for 72 h.The values of Olive tail moment in the 7.5 and 10 μmol/L groups were significantly higher than those in the solvent control (P ＜0.05).Moreover,the micronucleus rates in the 10 and 15 μmol/L groups were significantly higher than those in the solvent control (P＜0.05).The results of comet assay and micronucleus test showed a dose-response relationship.Conclusion:2,6-DCBQ exhibited strong cytotoxicity and induced DNA and chromosomal damage in NIH3T3 cells.关键词
drinking water/benzoquinone/cytotoxicity/genotoxicityKey words
drinking water/benzoquinone/cytotoxicity/genotoxicity分类
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Ying Xia,Xiao Wei..Acute andchronic cytotoxicity and genotoxicity induced by2,6-dichloro-1,4-benzoquinone in NIH3T3cells[J].广西医科大学学报,2018,35(3):281-285,5.基金项目
This work was supported by grants from National Natural Science Foundation of China (No.81560524,81360421),the China Postdoctoral Science Foundation (No.2013M540686,2014T70839),the Guangxi Natural Science Foundation (No.2012GXNSFBA053109),and the Outstanding Young Middle-aged Excellent Teachers' Training in Higher Education Institutions of Guangxi. （No.81560524,81360421）
