广西医科大学学报2018，Vol.35Issue(3)：286-289,4.DOI:10.16190/j.cnki.45-1211/r.2018.03.002
全骨髓贴壁法与密度梯度离心法培养小鼠内皮祖细胞的方法比较
Comparison of whole bone marrow culture method and density gradient centrifugation method of isolating mouse endothelial progenitor cells
摘要
Abstract
Objective:To compare the effects of whole bone marrow culture method and density gradient centrifugation method on isolating mouse endothelial progenitor cells (EPCs).Methods:Primary EPCs were obtained from male KM mice (8 weeks old) and isolated by whole bone marrow culture method and density gradient centrifugation method.Cell morphology,the abilities of uptaking DiI-acLDL and binding FITC-UEA-I,and cell proliferation were detected.Results:Primary cells isolated with whole bone marrow culture method showed round,short spindle shape or long spindle shape on day 4,and adherent cells mainly displayed similar circular shape and spindle shape on day 6 to 8.Primary cell cultured with density gradient centrifugation method showed round,polygonal and long spindle shapes,and "cobblestone" morphology.The immunofluorescence showed that double staining positive rate of DiI-acLDL and FITC-UEA-I in cells cultured with whole bone marrow culture method was higher compared with density gradient centrifugation method (P＜0.05),but no significant difference was found in cell proliferation rate between the two methods (P ＞0.05).Conclusion:The proliferation ability of EPCs cultured with whole bone marrow culture method and density gradient centrifugation method was similar,but the former was more easy to conduct and higher success rate.关键词
小鼠/内皮祖细胞/细胞培养/全骨髓贴壁法/密度梯度离心法Key words
mouse/endothelial progenitor cells/cell culture/whole bone marrow adherence method/density gradient centrifugation分类
医药卫生引用本文复制引用
赵文飘,戴霞,刘玉花,翟露,林媛,罗组纯..全骨髓贴壁法与密度梯度离心法培养小鼠内皮祖细胞的方法比较[J].广西医科大学学报,2018,35(3):286-289,4.基金项目
国家自然科学基金资助项目(No.81660147) （No.81660147）
