广西医科大学学报2018，Vol.35Issue(3)：290-293,4.DOI:10.16190/j.cnki.45-1211/r.2018.03.003
沉默大鼠颌下腺细胞SSB1对NBS1蛋白表达的影响
The effect of silencing single-stranded DNA-binding protein 1 on the expression of NBS1 protein in rats
摘要
Abstract
Objective:To investigate the protein expression of NBS1 after single-stranded DNA-binding protein 1 (SSB1) gene silencing.Methods:The submandibular gland (SMG) cells of rats were cultured and randomly divided into 3 groups:the virus group in which the cells were transfected with rAdE5-SSB1-1p2-shR-NA adenovirus expression vector,the blank control group in which the cells were transfected with empty adenovirus,and normal control group.The protein expressions of SSB1 and NBS1 were detected by western blotting.Results:The expressions of NBS1 and SSB1 in the virus group were significantly lower than those in the blank control group and normal control group (P＜0.05).Conclusion:SSB1 gene was successfully silenced in the SMG cells of rats.SSB1 silencing could down-regulate the protein expression of NBS1.关键词
SSB1/NBS1/腺病毒载体/免疫印迹/大鼠颌下腺细胞Key words
SSB1/NBS1/adenovirus vector/western blotting/submandibular gland cells分类
医药卫生引用本文复制引用
吕秋丽,陈龙,义彬玲,王代友..沉默大鼠颌下腺细胞SSB1对NBS1蛋白表达的影响[J].广西医科大学学报,2018,35(3):290-293,4.基金项目
国家自然科学基金资助项目(No.81560187) （No.81560187）
广西自然科学基金资助项目(No.2014GXNSFDA118022) （No.2014GXNSFDA118022）
