广西医科大学学报2018，Vol.35Issue(3)：302-305,4.DOI:10.16190/j.cnki.45-1211/r.2018.03.006
力生长因子对人外周血CD4+CD25+CD127-调节性T细胞体外增殖的影响
Effect of mechano growth factor on the proliferation of human peripheral blood CD4+ CD25+CD127-regulatory T cells
摘要
Abstract
Objective:To investigate the effect of mechano growth factor (MGF) on the proliferation of CD4+CD25+CD127-regulatoryT cells (Treg) in vitro.Methods:CD4+CD25+CD127 Treg cells in peripheral blood from healthy person were isolated by magnetic activated cell sorting (MACS).After purified,the cells were labeled with carboxyfluorescein (CFSE) and treated with different concentrations (0 nmol/L,10 nmol/L,100 nmol/L) of MGF.Treg cells proliferation was analyzed using flow cytometry.Peripheral blood mononuclear cells (PBMC) were isolated and treated with different concentrations (0 nmol/L,10 nmol/L,100 nmol/L) of MGF.The proportion of Treg cells in PBMC was detected by flow cytometry.Results:The cell proliferation rate was higher in 100 nmol/L MGF group than 0 nmol/L MGF group (P＜0.05).The proportion of Treg cells in PBMC was 17.82％±1.23％ in 0 nmol/L MGF groug,20.73％±1.61％ in 10 nmol/L MGF group,and 20.29％±1.46％ in 100 nmol/L MGF group.After treated with 10 nmol/L and 100 mol/L MGF,the proportion of Treg cells in PBMC was markedly increased,compared with 0 nmol/L MGF group (P ＜0.05),but no significant difference was noted between 10 nmol/L MGF group and 100 nmol/L MGF group (P＞0.05).Conclusion:MGF could promote the proliferation of CD4+ CD25+ CD127-Treg cells in vitro.关键词
调节性T细胞/力生长因子/体外扩增Key words
regulatory T cells/mechano growth factor/proliferation in vitro分类
医药卫生引用本文复制引用
周林华,林海,黄承宇,杨劲松..力生长因子对人外周血CD4+CD25+CD127-调节性T细胞体外增殖的影响[J].广西医科大学学报,2018,35(3):302-305,4.基金项目
广西自然科学基金资助项目(No.2017GXNSFAA198127) （No.2017GXNSFAA198127）
