广西医科大学学报2018，Vol.35Issue(3)：306-310,5.DOI:10.16190/j.cnki.45-1211/r.2018.03.007
妊娠期齐多夫定干预对新生儿、新生鼠造血干/祖细胞活性的影响
Influence of zidovudine administration during pregnancy on the activity of haematopoietic stem/progenitor cells in neonatus and neonatal mice
摘要
Abstract
Objective:To investigate the influence of zidovudine(AZT) administration during pregnancy on the activity of haematopoietic stem/progenitor cells(HSPCs) in neonatus and neonatal mice.Methods:Cord blood from 15 HIV+ mothers(received highly active antiretroviral therapy,HAART,AZT+3TC+LPV/r) and 12 HIV-uninfected mothers were collected.The proportion of CD34+ cells was detected by flow cytometry.The formation of HSPCs colonies including granulocyte-monocyte colony forming units (CFU-GM),burst forming unit-erythroid(BFU-E) and colony forming unit-megakaryocyte(CFU-Meg) was calculated.Umbilical cord blood HSPCs and bone marrow cells of neonatal mice exposed to AZT were isolated and cultured in vitro.The CFU-GM,BFU-E and CFU-Meg colonies and the apoptosis rate of HSPCs were observed.Results:HAART exposure resulted in a decreased rate of CD34+ cells,and decreased number of colonies including CFU-GM,BFU-E and CFU-Meg(P＜0.05).After treated with 1.0 μmol/L AZT,the number of colonies including CFU-GM,BFU-E and CFU-Meg were significantly reduced(P＜0.05).The apoptosis rate in cord blood HSPCs and bone marrow cells of neonatal mice was increased by treatment of AZT(＞0.5 μmol/L).Administration of AZT at different doses during pregnancy could inhibit the colony formation of CFU-GM,BFU-E and CFU-Meg(P＜0.01).Conclusion:Administration of AZT during pregnancy could suppress the activity of HSCPs.关键词
齐多夫定/脐带血/骨髓造血干/祖细胞/活性/凋亡Key words
zidovudine/umbilical cord blood/hematopoietic stem/progenitor cells/activity/apoptosis分类
医药卫生引用本文复制引用
陈悦,邝晓聪,李慕军,曾雅畅,罗敏玲,盘兰姣..妊娠期齐多夫定干预对新生儿、新生鼠造血干/祖细胞活性的影响[J].广西医科大学学报,2018,35(3):306-310,5.基金项目
广西壮族自治区卫计委重点课题资助项目(No.桂卫重2010007) （No.桂卫重2010007）
