广西医科大学学报2018，Vol.35Issue(3)：311-314,4.DOI:10.16190/j.cnki.45-1211/r.2018.03.008
三七皂苷R1对高糖诱导HK-2细胞转分化的作用及其机制研究
Effect and mechanisms of Notoginsenoside R1 on high glucose induced epithelial-mesenchymal transition in HK-2 cells
摘要
Abstract
Objective:To investigate the effect and mechanisms of Notoginsenoside R1 (NTR1) on high glucose induced epithelial-mesenchymal transition (EMT) in renal tubular epithelial cells (HK-2).Methods:HK-2 cells were divided into 4 groups:control group,mannitol group,high glucose group,and high glucose + NTR1 group.The cells were cultured for 48 h.The drug concentration was screened by MTT.The expressions of nuclear factor-κB (NF-κB) and tumor necrosis factor (TNF)-α were determined by western blotting.The levels of α-smooth muscle actin (α-SMA) and fibronectin (FN) were detected by enzyme linked immunosorbent assay (ELISA).Results:The safe dose of NTR1 was below 60 μmol/L.The protein expressions of NF-κB p65,TNF-α,α-SMA and FN in high glucose group and high glucose + NTR1 group were significantly higher than those in the control group and mannitol group (P ＜0.05),but no significant difference was found between the control group and mannitol group (P ＞ 0.05).Compared with the high glucose group,the expression of NF-κB p65,TNF-α,α-SMA and FN in high glucose + NTR1 group was notably decreased (P ＜0.05).Conclusion:NTR1 could inhibit high glucose induced EMT in HK-2 cells,alleviate inflammatory response,and attenuate renal interstitial fibrosis.The mechanisms might be related to the suppression of NF-κB/TNF-α signaling pathway.关键词
糖尿病肾病/上皮细胞间质转化/三七皂苷R1/核因子-κB/肿瘤坏死因子-αKey words
diabetic nephropathy/epithelial-mesenchymal transition/Notoginsenoside R1/NF-κB/TNF-α分类
医药卫生引用本文复制引用
依超,温海滨,覃勋,王洁,林栩,胡美玲,蒙如庆..三七皂苷R1对高糖诱导HK-2细胞转分化的作用及其机制研究[J].广西医科大学学报,2018,35(3):311-314,4.基金项目
2016年广西研究生教育创新计划课题资助项目(No.201601008) （No.201601008）
