广西医科大学学报2018，Vol.35Issue(3)：315-318,4.DOI:10.16190/j.cnki.45-1211/r.2018.03.009
法舒地尔对自发高血压大鼠血压的作用及其机制研究
The effect and mechanism of fasudil on spontaneous hypertension in rats
摘要
Abstract
Objective:To investigate the effect and mechanisms of fasudil on spontaneous hypertension (SHR) in rats.Methods:A total of 30 SHR rats were randomly divided into 5 groups (n =6 per group):model group,nifedipine group (10 mg/kg nifedipine via gavage),and fasudil low-,medium-and high-dose groups (respectively 5 mg/kg,10 mg/kg and 20 mg/kg fasudil via gavage).Six healthy WKY rats were selected as a normal control group.The rats in the model group and the normal control group were given equal volume of saline via gavage.The systolic caudal arterial pressure,left ventricular mass index (LVWI),the serum levels of nitric oxide (NO) and endothelin-1 (ET-1) as well as the mRNA expressions of RhoA and Rho kinase in myocardium,kidney and aorta were detected.Results:The systolic caudal arterial pressure was significantly decreased by treatment of nifedipine or fasudil for 2,4,6 and 8 weeks (P＜0.05),and was lower in high-dose fasudil group compared with nifedipine group and medium-dose fasudil group (P＜0.05).After treatment,the systolic caudal arterial pressure,NO;evel,LVWI and the mRNA expressions of RhoA and Rho kinase in myocardium,kidney and aorta from lowest to highest as follows:normal control group＞ high-dose fasudil group＞ medium-dose fasudil group＞ nifedipine group＞ low-dose fasudil group＞ model group,while the serum ET-1 level was the opposite.There were significant differences among groups (P＜ 0.05).Conclusion:Fasudil could effectively reduce the systolic caudal arterial pressure in SHR rats,and was dose-dependent in a certain,the mechanisms might be associated with the reduced expressions of RhoA and Rho kinase,and regulation of serum NO and ET-1 levels.关键词
法舒地尔/自发高血压/左心室重量指数/作用机制Key words
fasudil/spontaneous hypertension/left ventricular mass index/mechanism分类
医药卫生引用本文复制引用
范彦夫,张志英,韩秀红,杜淑华,齐建祥,邵伟然,赵秀芹,王春敏..法舒地尔对自发高血压大鼠血压的作用及其机制研究[J].广西医科大学学报,2018,35(3):315-318,4.基金项目
佳木斯大学科学技术校级面上项目(No.13Z1201557) （No.13Z1201557）
