中医学报2018，Vol.33Issue(3)：440-444,5.DOI:10.16368/j.issn.1674-8999.2018.03.106
中药复方联合运动疗法对CHF大鼠MMP-2及胶原蛋白的影响
Effects of Chinese Herbal Compound Prescription Combined with Exercise Therapy on MMP-2 and Collagen in CHF Rats
摘要
Abstract
Objective:To explore the curative effect of Chinese herbal compound prescription combined with exercise therapy on chronic heart failure (CHF) and to analyze its target of action.Methods:Rat model of CHF was established by ligation of coronary arteries.According to body weight,the rats were randomly divided into TCM group,western medicine group,model group,TCM plus exercise group and exercise group.The heart function was analyzed by ultrasound,and the expression of matrix metalloprotein ase-2 (MMP-2) and tissue inhibitor of metalloproteinase (TIMP-2) was examined with real-time fluorescence quantitative PCR method.Results:The levels of MMP-2 in Chinese medicine group and plus exercise group and western medicine group were (1.212 ± 0.146) and (1.605 ± 0.264) respectively by real-time fluorescence quantitative PCR,and there was significant difference between the two groups (P≤0.001).The levels of TIMP-2 of the western medicine group was (1.519 ± 0.269) and that of the Chinese medicine group was (1.327 ± 0.246);there was significant difference between the two groups (P =0.009).The levels of TIMP-2 of Chinese medicine plus exercise group was (1.839 ± 0.324),which was significantly better than that of the western medicine group (P≤.001).The type Ⅰ collagen in Chinese medicine group was (1.013 ±0.226) and that in western medicine group was (0.872 ±0.182),and the difference was statistically significant (P =0.031).The levels of TIMP-2 of the exercise group was (1.136 ± 0.251),which was significantly better than that of the western medicine group (P ≤ 0.001).Conclusion:The effect of combination of Chinese herbal compound prescription and exercise therapy in treatment of chronic heart failure rats is significant,which can improve the myocardial remodeling in CHF rats.关键词
中药复方/运动疗法/心力衰竭/MMP-2/胶原蛋白Key words
Chinese herbal compound prescription/exercise therapy/heart failure/MMP-2/collagen分类
医药卫生引用本文复制引用
礼海,马金,张洋,张艳..中药复方联合运动疗法对CHF大鼠MMP-2及胶原蛋白的影响[J].中医学报,2018,33(3):440-444,5.基金项目
辽宁省沈阳市科技局资助项目(F13-316-1-10) （F13-316-1-10）
