哈尔滨商业大学学报（自然科学版）2018，Vol.34Issue(2)：129-133,158,6.
爪哇黄芩二氢黄酮对HepG-2细胞增殖的影响
Effects of flavonone in Scutellaria javanica Jungh.on HepG-2 cells proliferation
摘要
Abstract
To study the anti -tumor activities effects of flavanone in Scutellaria javanica Jungh.,active fraction and sensitive cell line were selected.The effect on HepG-2 cell proliferation was researched to reveal the anti-tumor activities of flavanone in Scutellaria ja-vanica Jungh.This paper provided the experimental foundation for develop natural drugs. The human liver cancer cell HepG-2, gastric cancer cell SGC -7901, lung cancer cell A549,breast cancer cell MCF-7 and C6 glioma cells, cultivated in vitro, were used as models in the paper.With median inhibitory concentration(IC50)used as index,MTT col-orimetry was applied to ascertain the anti-proliferative effects of petroleum ether, dichlo-romethane and n-butyl alcohol extraction fractions in Scutellaria javanica Jungh.on the a-bove-mentioned 5 cancer cells.The dichloromethane extracted fraction of Scutellaria javan-ica Jungh.was its active fraction.HepG-2 cells is its sensitive cell line.The MTT colori-metry was adopted to ascertain the inhibiting effects of flavanone monomers extracted from the active fractions on human liver cancer cell HepG-2 to screen active monomers.The results suggested that the 5,6,2′-trihydroxy-7 -methoxy flavanone IC50of Scutellaria javanica Jungh.was 110.3 μmol/L,and the 5-hydroxyl-6,7,2′,6′-tetramethyl flavanone IC50 was 747.21 μmol /L.It was discovered that the 5,6,2′-trihydroxy-7-methoxy flavanone was the active composition of Scutellaria javanica Jungh.on human liver cancer cell HepG-2.关键词
爪哇黄芩/二氢黄酮/人肝癌细胞HepG-2/细胞增殖/抑制作用Key words
Scutellaria javanica Jungh./flavanone/HepG-2 cell/cell proliferation/inhib-iting effects分类
医药卫生引用本文复制引用
于蕾,姜波,王鑫,杨经辉..爪哇黄芩二氢黄酮对HepG-2细胞增殖的影响[J].哈尔滨商业大学学报（自然科学版）,2018,34(2):129-133,158,6.基金项目
黑龙江省普通高等学校肿瘤预防与抗肿瘤药物研究重点实验室开放课题(CPAT-2012003) （CPAT-2012003）
哈尔滨市科技局创新人才项目(2014RFQXJ154) （2014RFQXJ154）
哈尔滨商业大学博士科研启动项目(12DL008) （12DL008）
哈尔滨商业大学科研团队计划(项目号:2016TD002) （项目号:2016TD002）
哈尔滨商业大学青年创新人才支持项目(2016QN057) （2016QN057）
