华西口腔医学杂志2018，Vol.36Issue(2)：150-155,6.DOI:10.7518/hxkq.2018.02.007
与重症低龄儿童龋病相关的唾液微生物群落研究
Salivary microbial communities associated with severe early childhood caries
摘要
Abstract
Objective To compare the salivary microbial profiles of healthy subjects and those with severe early childhood caries (S-ECC) by using high-throughput sequencing. Methods Salivary samples were obtained from children with S-ECC (group C, n=24) and healthy children (group H, n=24). Total metagenomic DNA was extracted, and DNA amplicons of the V1-V3 hypervariable region of the 16S rRNA gene were generated and subjected to 454 sequencing. The characteristics of oral microbial communities from the two groups were compared based on microbial diversity and taxonomy assignment. Results First, the microbial richness was significantly higher in group C than group H (P<0.05). Second, the microbial com-munity structure was significantly different for the groups H and C (P<0.01). In addition, caries microbiota was significantly conserved in group C (P<0.001). High expression of suspected cariogenic microorganisms in group C (P<0.1) and health related microorganisms in group H (P<0.1) were identified. Finally, models of caries risk assessment were proposed to distin-guish caries from healthy subjects with over 70% accuracy. Conclusion Salivary microbiota and certain taxa, such as caries-associated taxa(Prevotella),may be useful to screen/assess the children's risk of developing caries.关键词
低龄儿童龋/口腔菌群/唾液/元基因组学Key words
early childhood caries/oral microbiota/saliva/metagenomic分类
医药卫生引用本文复制引用
孙同正,滕飞,贾松菠,唐永平,姜明,黄适,袁晓,李晓岚,杨芳..与重症低龄儿童龋病相关的唾液微生物群落研究[J].华西口腔医学杂志,2018,36(2):150-155,6.基金项目
国家自然科学基金(81670979,31600099) （81670979,31600099）
青岛市自助创新计划(应用基础研究项目-青年专项)(16-5-1-67-jch) （应用基础研究项目-青年专项）
广东省口腔医学重点实验室开放课题(KF2016120101)The National Natural Science Foundation of China(81670979,31600099) （KF2016120101）
The Self-help Innovation Plan of Qingdao(Application of Basic Research Project-Youth Special)(16-5-1-67-jch) （Application of Basic Research Project-Youth Special）
Open Fund of Guangdong Provincial Key Laboratory of Oral Diseases(KF2016120101). （KF2016120101）
