军事医学2017，Vol.41Issue(12)：947-951,5.DOI:10.7644/j.issn.1674-9960.2017.12.001
泛素连接酶Smurf1促进PDK1的多聚泛素化修饰
Ubiquitin ligase Smurf1 promotes poly-ubiquitination of PDK1
摘要
Abstract
Objective To investigate the mechanism of 3-phosphoinositide-dependent protein kinase 1(PDK1)poly-ubiquitination.Methods Co-immunoprecipitation(Co-IP)and Western blot(WB)were used to analyze poly-ubiquitination of PDK1.It was confirmed that ubiquitin ligase smad ubiquitylation regulatory factor 1(Smurf1)inprove PDK1 poly-ubiquitination within MEF cells,site-directed mutagenesis and WB before PDK1 poly-ubiquitination sites were determined.Results We found that PDK1 could undergoes poly-ubiquitination,ubiquitin ligase Smurf1 was found to be a direct E3 ligase for PDK1 poly-ubiquitination and might rely on the ubiquitin ligase Smurf 1 K699 site activity.K304 was PDK1 poly-ubiquitination modification site point.Conclusion The ubiquitin ligase Smurf1 can promate poly-ubiquitination of PDK1.关键词
泛素连接酶/泛素化修饰/免疫共沉淀/免疫印迹法/Smurf1/PDK1/PDPK1/细胞增殖/质粒/突变Key words
ubiquitin ligases/ubiquitination/immunoprecipitation/immunoblotting/Smurf1/PDK1/PDPK1/cell proliferation/plasmids/mutation分类
医药卫生引用本文复制引用
魏军,时慧森,党智笙,张令强,张学利..泛素连接酶Smurf1促进PDK1的多聚泛素化修饰[J].军事医学,2017,41(12):947-951,5.基金项目
国家自然科学基金资助项目(81772959) （81772959）
上海市科委实验动物研究领域项目(16140902000) （16140902000）
