军事医学2017，Vol.41Issue(12)：952-955,961,5.DOI:10.7644/j.issn.1674-9960.2017.12.002
Abro1在LPS诱导的急性肺损伤中的作用探讨
Role of Abro1 in LPS-induced acute lung injury
摘要
Abstract
Objective To investigate the effect of Abro1 on acute respiratory distress syndrome(ARDS)/acute lung injury(ALI)in mice.Methods Abro1 knock-out(KO)mice and wild type(WT)mice were both randomly divided into two groups for intratracheal instillation of lipopolysaccharide(LPS)or normal saline.At 6 or 24 hours after treatment, the pathological changes in lung tissue were observed by HE staining.At 6 hours after treatment,inflammatory immune cells and cytokines production(IL-6)in the bronchoalveolar lavage fluid were examined.Myeloperoxidase(MPO)and the mRNA level of IL-6 in the lung tissue were compared.Results At 24 hours after treatment, compared with WT mice treated with LPS,Abro1 KO mice showed a significantly lower lung injury score.At 6 hours after treatment,Abro1 depletion resulted in reduced levels of inflammatory immune cell infiltration and cytokines production(IL-6)in the bronchoalveolar lavage fluid(P<0.05).In addition,the MPO content and the mRNA level of IL-6 in the lung tissue were much lower than those in WT mice treated with LPS for 6 hours(P<0.05).Conclusion Abro1 deficiency can attenuate LPS-induced ARDS/ALI.关键词
呼吸窘迫综合征,成人/急性肺损伤/脂多糖类/小鼠,基因敲除/Abro1/支气管肺泡灌洗液Key words
respiratory distress syndrome,adult/ALI/LPS/mice/knockout/Abro1/bronchoalveolar lavage fluid分类
医药卫生引用本文复制引用
陈姣姣,孙飞飞,詹轶群,葛常辉,杨晓明..Abro1在LPS诱导的急性肺损伤中的作用探讨[J].军事医学,2017,41(12):952-955,961,5.基金项目
国家"973"计划资助项目(2013CB910803) （2013CB910803）
